The fast and accurate reversed-phase HPLC method was predicated on qualitative and quantitative estimation of the total concentrations of the essential thiols in the plasma of adults volunteers compared with epileptic patients, which is l-cysteine (Cys) total homocysteine (tHys), cysteinyl glycine (Cys-Gly), glutathione (GSH) and cysteamine (Cstm), respectively. The plasma samples of (50) epileptic patients were classified into three groups; the non-anti-epileptic drug consisted of 20 patients (12 male, eight female) who were mainly diagnosed with idiopathic generalized epilepsy. The mean age was 34 ± 13 (range 19–48) years. The second group consisted of 30 patients receiving two types of anti-epileptic drugs, 18 patients (12 male and six female) receiving Carbamazepine, and 12 patients (7 males and five female) receiving phenytoin. The mean age of the patients was 32 ± 11 Compared with 31 healthy volunteers (20 male and 11 female). The mean age of the controls was 31 ± 9 (range 26–49 years). The mean duration of treatment of patients receiving the anti-epileptic drug was six months). All measurement was done using pre-column derivatization with bromobimane, and the derivative of each standard mixture were baseline separated on C18-DB (50 x 4.6 mm ID) column, 3μm particle size. The regression coefficients for the separated and standard deviation SD within-run ranged from 0.09 to 8.40 μmol/L, and between-run ranged from 0.15to 9.16 μmol/L; the analytical procedure gave good linearity in the range between 1.25 to 20 µmole/ l, the detection limit was 0.1 µmole /L for all the thiol groups. Analytical recovery was 96.9–107.4 %; the mean concentration of plasma cysteine and total homocysteine was slightly higher in males than females; it was 221± 75 for adult males and 190± 44 μmol/L for adult females, while t-homocysteine was (10.55 ±2.45 vs. 9.79 ± 1.88 μmol/L, the results observed that the value of cysteine and homocysteine were significantly higher in epileptic patients using Carbamazepine and phenytoin than in healthy volunteers. Mean values for glutathione were lower, while cysteinyl glycine showed no significant difference in healthy and epileptic patients and no sex- and age-dependent.

The objective of the present study is to determine the anti-obesity effects of Akkermansia munciniphila  on some biometric measurements. 40 patients were divided into two groups, each with 20 patients; G1 group consumed the probiotic of A. munciphila   and G2   consumed placebo capsules. The results  showed significant effects on the body weight (p≤ 0.001), which was reduced by a range of -2.29 to -0.7, whereas non-significant differences were observed in the Waist circumferance  . The study also showed significant differences (p≤0.001) between the two groups in the levels of cholesterol (287 vs. 285 mg/dl) , whereas the level of  Triglyceride  significantly  decreased  in the treated patients (p≤0.001). No significant differences were observed in the concentration of fat.  There were also  significant differences in the levels of fetuin-A  (p≤0.020) and serotonin ( p≤ 0.039 ).  The consumption of the probiotic of A. muciniphila  was proved to be beneficial for human health, while further studies are needed to explore more effects.

PCOS syndrome is major compatible female problem there for this mini review was focus on the research which are tested the effect of probiotic supplement on PCOS. Methods: In this mini review the was key word such as (PCOS, obesity, lipid profile, testosterone) and used Google scholar and pub med. the study was used 7 article published between (2015-2019). Results: The study was positive result about effect of probiotic on the body weight, lipid profile, sex hormone and oxidative factors. the study was showed more relationship between the 7 articles which are used in this study. Conclusion: This review was showed probiotic positive function on PCOS treatment in the women.

The present study was undertaken to investigate the susceptibility patterns as antifungal and growth of certain D-amino acids inhibitory effects, includes D-alanine(D-ala) and D-proline (D-pro). The obtained results indicated that D-proline acid is potent mostly as antifungal, among tested D-amino acids, then followed by D-ala. The study aim was evaluating the of D-amino acids and nanoparticles against Candida albicans adhered cells and bio-films. Results showed that D-ala MIC value was 25 μg/ml, while in methods of TCP, (D-pro) MIC 50 μg/ml value. Nanoparticles (lithium and silver) and amino acids (D-pro and D-ala) effect in preventing cells adhesion on polystyrene surface and mature bio-film inhibition was studied. Highest inhibition was obtained at D-pro concentration 50 μg /ml, while the lowest at concentration D-ala 25 μg /ml against mature bio-film and cell adhesion.

TORCH syndrome is only one health issue. According to health experts, however, TORCH syndrome is a set of various infectious diseases. They can cause serious problems and can damage a fetus' health. Aim of study: To detection the causative agent of abortion in Babylon city –Iraq by using TORCH test . Material and patient : Hundred ninety five women severed from abortion were enrolled in this study .The duration of study continuous from July 2018-to June 2019. ELISA test was used for determination the titter of IgG, IgM form all participat. Result :The study result was showed positive casesfor IgM, IgG of Toxoplasma (94,84) respectively with percentage (57.3%,54.2%). While negative case was showed (12,22) respectively with percentage (38.7%, 55%) .The study was showed in CMV positive titter case in botheIgM,IgG(65,75 ) respectively with percentage( 69.1%, 60%) .while the negative case was (40,31) respectively with present (40%,44.2%) In Rubella positive IgM, IgG was register as (19,31) with present (73% ,34%).while negative IgM,IgG was (87,75) respectively with percentage (51%,71%). Finally the positive result of IgM, IgG HSV was showed (6,31) respectively with percentage (54.5 %&53%) while the negative result is (79,65) respectively with present (43%& 46%). Conclusion : this study was found ,the toxoplasma was the major factor for the abortion more than the viruses ,we need more study to determination of the relationship between the abortion and infection of viruses .