This study aims to evaluate the 5-alpha reductase enzyme (SRD5A2) levels in serum and it’s association with some sex hormones such as Follicle stimulating hormone (FSH), Luteinizing hormone (LH), prolactin and testosterone hormone and the infertility type in some infertile Iraqi men. Blood and seminal fluid samples from (60) idiopathic male infertile and (30) healthful individuals as a control group aged (18 to 60 year) are collected from private clinics. Enzyme Linked Immunosorbent Assay (ELISA) has been used for estimation of serum SRD5A as enzymes and FSH, LH, prolactin and testosterone as hormones.According to types of sperm count patients have been divided to two group 49 person as azoospermia group (zero /ml) and 11 person as oligospermia group less than 20 million/ml. The levels of SRD5A2 and testosterone are significantly decreased to (P≤0.01) in the infertile men as compared with control group. While the level of FSH, LH and prolactin are significantly higher (P≤ 0.01) in the infertile men than control group. The results of SRD5A2 are non-significant, while, levels of FSH, LH and prolactin are higher significant (P≤ 0.01) except testosterone level which has significant differences (P≤ 0.05) in the age groups <20-30 year, 31–40 year and ≥ 40 year. There are significant differences (P≤ 0.01) in levels of SRD5A2, FSH and prolactin, but the levels of LH are non-significant in the infertility period ≤ 10 year, 11 – 20 year, ≥ 20 year. Smoker infertile men have low levels (P≤ 0.01) in the SRD5A2 and prolactin while have high levels in the LH hormones compared with control. Patients with family history have shown significant differences (P≤0.05) in the levels of SRD5A2, testosterone, LH and prolactin. In conclusion, this study revealed significantly decrease in the levels ofSRD5A2 in the Azoospermic and oligospermic infertile men and significant negative correlation (P<0.05) between SRD5A2 pg/ml and FSH (mIu/ml) R factor-0.328. Therefore, SRD5A2 has important role in the diagnosis of idiopathic male infertility and it's one of the important markers in the diagnosis of normal spermatogenesis.

The aim of the current study to determine levels of Inhibin-B hormone and its relationship with some gonadal hormones, including follicle-stimulating hormone (FSH), luteinizing hormone (LH) prolactin, and testosterone hormones in some males with idiopathic infertility in Shirqat city.  Blood and seminal fluid samples from (60) idiopathic male infertile and (30) healthful individuals as (control group) aged (18 to 60 years) were collected from private clinics. Enzyme-linked immunosorbent assay (ELISA) was used to estimate serum Inhibin B, FSH, LH, prolactin, and testosterone hormones. According to types of sperm count, patients have divided onto two groups 49 persons as azoospermia group (zero /ml) and 11 persons as oligospermia group less than 20 million/ml. Inhibin B and testosterone levels were significantly decreased (P≤0.01) in the infertile men compared with the control group. While the FSH level, LH, and prolactin were significantly higher (P≤ 0.01) in the infertile men than in the control group, the results of Inhibin B were non-significant. In contrast, levels of FSH, LH, and prolactin were higher significant (P≤ 0.01), except testosterone level was significant differences (P≤ 0.05) in the age groups <20-30 year, 31–40 year, and ≥ 40 years. There were significant differences (P≤ 0.01) in levels of FSH and prolactin, but the levels of Inhibin B and LH were non-significant in the infertility period ≤ 10 year, 11 – 20 years, ≥ 20 years. Smoker infertile men have low levels (P≤ 0.01) in the prolactin while had high levels in the Inhibin B and LH hormones compared with control. Patients with a family history showed a significant difference (P≤0.05) in the levels of Inhibin B, testosterone, LH, and prolactin.  In conclusion, this study revealed a significant decrease in the Inhibin-B levels of the Azoospermic and oligospermia infertile men. The negative correlation between inhibin B and testosterone and weak correlation with other gonadal hormones. Therefore, Inhibin-B has an important role in diagnosing idiopathic male infertility, and it's one of the important markers in the diagnosis of normal spermatogenesis.