ObjectiveThis experiment was conducted to investigate the effects of dietary crude protein (CP) level and exogenous protease supplementation on growth performance, serum metabolites, carcass traits, small intestinal morphology and endogenous protease activity in broiler chickens reared under a tropical climate.MethodsA total of 480 day-old male broiler chicks were randomly assigned to eight dietary treatments in a 4×2 factorial arrangement. The main effects were CP level (21.0%, 19.7%, 18.5%, or 17.2% from 1 to 21 days and 19.0%, 17.9%, 16.7%, or 15.6% from 22 to 35 days) and protease enzyme supplementation (0 ppm or 500 ppm). All experimental diets were fortified with synthetic feed-grade lysine, methionine, threonine and tryptophan to provide the minimum amino acid recommended levels for Cobb 500.ResultsReducing dietary CP linearly reduced (p<0.05) growth performance, serum albumin, total protein, and carcass traits and increased (p<0.05) serum triglycerides and abdominal fat. There was no consistent effect of reducing dietary CP on morphological parameters of the intestine and on the pancreatic and intestinal endogenous protease activity (p>0.05). Protease supplementation improved (p<0.05) feed conversion ratio, body weight gain, carcass yield and intestinal absorptive surface area.ConclusionProtease supplementation, as measured by growth performance, intestinal morphology and carcass yield, may alleviate the detrimental effects of low protein diets in broiler chickens.

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The effects of feeding low dietary crude protein (CP) and/or metabolisable energy (ME) with or without supplemental protease on growth performance, carcase characteristics and physiological responses in broiler chickens were investigated under cyclic heat stress condition. A total of 350 day-old male broiler chicks were fed with one of the following seven experimental diets: (1) recommended-CP and recommended-ME (RPE, served as control); (2) recommended-CP and low-ME (RPLE); (3) recommended-CP and low-ME with protease (RPLEP); (4) low-CP and recommended-ME (LPRE); (5) low-CP and recommended-ME with protease (LPREP); (6) low-CP and low-ME (LPE) and (7) low-CP and low-ME with protease (LPEP). From 22 to 42 d of age, half of the chickens from each dietary group were exposed to 34 ± 1 C for 7 h daily (heat stress), whereas the other half were raised at constant 23 ± 2 C (normal temperature). Supplementation of protease to RPLE, LPRE and LPE diets had no significant effects on feed intake (FI), weight gain (WG) or feed conversion ratio (FCR). Diet had no effect on serum glucose, total protein, certain acute phase proteins (APPs), corticosterone or breast yield. Regardless of protease supplementation, heat stressed birds had significantly lower FI, WG and breast yield, and higher FCR, APPs and corticosterone compared to birds raised in normal temperature. In conclusion, dietary supplementation of protease to low CP and/or ME diets showed negligible effects on growth performance, carcase characteristic and physiological responses in broiler chickens under heat stress condition. The inclusion of microbial protease in broiler diets could be considered by poultry industry as an effective nutritional tool for reducing ME or CP, in order to decrease abdominal fat deposition, improve feed efficiency and increase the profit margin. HIGHLIGHTS Protease supplementation has no specific help for broilers under heat stress. Feeding low CP and/or ME diet is not stressful for broiler chickens.

The effects of protease supplementation on productive performance and physiological responses were investigated in broiler chickens fed reduced crude-protein (low-CP) diets and reared under normal or high stocking densities (SD). Chicks were housed in pens with two stocking densities (≈10 birds/m2 or ≈15 birds/m2) and fed diets with either the recommended or reduced level of CP and metabolisable energy (ME) from 1 to 21 days. From 22 to 42 days, an equal number of birds from each dietary group was fed one of the following finisher diets: (1) recommended-CP and ME, (2) low-CP and recommended-ME or (3) low-CP and recommended-ME supplemented with protease. No interactions (P &gt; 0.05) were observed between diet and SD for feed intake (FI), weight gain (WG), feed conversion ratio (FCR), Newcastle disease antibody titre, pododermatitis, caecal clostridia, Escherichia coli, lactobacilli, serum corticosterone or acute-phase proteins. Diet had no effect (P &gt; 0.05) on FI, WG or FCR during the finisher period nor on FI and WG during the overall (1–42 days) period. High SD resulted in lower FI (P &lt; 0.05) and Newcastle disease antibody titre (P &lt; 0.05) and higher FCR (P &lt; 0.05), pododermatitis incidence (P &lt; 0.05), caecal population of clostridia (P &lt; 0.05) and E. Coli (P &lt; 0.05), and acute-phase protein and corticosterone concentrations than did normal SD. In conclusion, the supplementation of protease to a low-CP diet during the finisher period had no effect on the performance and immunity of overcrowded broiler chickens. Regardless of dietary CP and protease supplementation, a high SD negatively affected the growth performance, immunity, caecal microflora and welfare of the broiler chickens.