The completion of the Three Gorges project altered the water level fluctuations from "summer submersion and winter exposure" to "summer exposure and winter submersion." The reverse seasonal flooding has dramatically changed both the time and duration of exposure and submergence of reservoir banks. Secondary seed dispersal by water is the primary method of dispersal in hydro-fluctuation belts. This study conducted field sampling and germination experiments to evaluate the effects of reverse seasonal flooding on species composition, diversity, and density of the secondary seed dispersal system and the soil seed bank, and their changes with elevation, in the Xiangzi River. The results indicate that that the secondary seed dispersal system and soil seed bank were associated with 42 and 50 plant species, respectively, and their average seed densities were 1,876.27 and 7,322.18 seeds/m 2 , respectively.The quantity of seeds from secondary seed dispersal accounted for 20.40% of the soil seed bank. The species life-form composition of both consisted mainly of annual and perennial grasses. Among these grasses, the dominant species in the secondary seed dispersal system were Cynodon dactylon and Paspalum distichum, whereas the dominant species in the soil seed bank were Solanum nigrum and Rumex dentatus. Due to the effects of reverse seasonal water level fluctuations, the species composition and quantity of the soil seed bank and secondary seed dispersal system largely exhibited similar spatial distribution characteristics, but some differences were observed. The species quantity, species diversity index, and seed density in the soil seed bank were the highest in the middle section of the hydro-fluctuation belt, followed by the top section and then the bottom section. Conversely, the species quantity, species diversity index, and seed density in the secondary seed dispersal system were highest at the top section, followed by the middle section and then the bottom section.