The goal of the current study was to determine the appropriate amount of yeast extract and level of nitrogen fertilization on growth, yield, and yield components of sesame (cv. shandaweel-3) under drip irrigation system. The investigation was conducted in the experimental farm of the faculty of agriculture and natural resources at Aswan University in the Aswan governorate during the summers of 2020 and 2021. Three replications were employed in the experiment, which was set up using a strip-plot layout and randomized complete block design (RCBD). the main plots were set up using yeast extract, and the sub-plots had nitrogen fertilization. the experiment included two factors: 1-yeast extract: no spray (control), One spry after 20 days from planting and tow spry after 20 and 30 days from planting. 2-Nitrogen fertilization: 30,45 and 60 unit / fed. The results showed that the studied yeast extract and their interaction (yeast extract + nitrogen fertilization) had no significant effects on the majority of the traits. These traits included plant height, frouting zone, number of capsules per plant, 1000-seed weight (g), seed weight plant -1 (g), seed yield kg fed -1 , seed oil %, and seed protein %. In addition, nitrogen fertilization levels at 60 units per fed had significant or highly significant effects on the majority of the traits.