Background: This study was conducted to evaluate working posture of operating room nurses and its relationship with demographic and job details of this group. Methods: This cross-sectional study was conducted among 147 operating room nurses in Tabriz, Iran using a questionnaire and the Rapid Entire Body Assessment (REBA) checklist. The data were analyzed with SPSS.16 using t test, Pearson correlation coefficient and analysis of variance (ANOVA) tests for univariate analysis and the linear regression test for multivariate analysis. Results: The mean (SD) of REBA score was 7.7 (1.9), which means a high risk level and highlights an urgent need to change the working postures of the studied nurses. There was significant relationship between working posture and age (P = 0.003), gender (P = 0.003), regular daily exercise (P = 0.048), work experience (P = 0.003), number of shifts per month (P = 0.006) and type of operating rooms (P < 0.001) in univariate analyses. Gender and type of operating room were the predictors of working posture of nurses in multivariate analysis. Conclusion: The findings highlight the need for ergonomic interventions and educational programs to improve working posture of this study population, which can consequently lead to promotion of health and well-being of this group.