Liver disorders are one of the principal reasons for mortality in the world. Isotretinoin is a systemic retinoid that has been approved for therapy of acne vulgaris since 1982. This drug causes complications in the body. Evidence suggests that Isotretinoin might cause hepatotoxicity. Our research aimed to study the exact mechanism of hepatotoxicity induced by isotretinoin and the protective role of taurine in this toxicity. Biomarkers such as aspartate transaminase (AST) and alanine transaminase (ALT), superoxide dismutase, glutathione content (GSH), catalase, and malondialdehyde (MDA) were examined. Furthermore, pathological changes were evaluated. The results showed that oral administration of Isotretinoin induced hepatotoxicity as showed by elevation in ALT, AST, and MDA; also, it reduced intracellular GSH in rat liver tissue. Administration of taurine prevented the hepatotoxicity induced by isotretinoin in rats significantly.