Plum pox virus (PPV), the causal agent of Sharka disease, causes yield, quality, and economic losses in stone fruits. PPV has been reported worldwide, especially in Europe. In studies to date, the presence of the virus has been identified as being restricted in different regions of Turkey. However, there is no record of PPV in Bolu province so far. Hence, surveys were carried out in Bolu province between 2016-2019, and a total of 306 samples were collected. To determine the presence of PPV, the samples were first tested by DAS-ELISA, and only three peach samples were found to be infected. DAS-ELISA results of infected samples were confirmed by RT-PCR using universal primers (P1/P2), then infected samples were identified at the strain level using strain-specific primers. The samples were found to be infected with the PPV-M (Marcus) strain and 243-bp long nucleotide sequences containing the partial coat protein gene of three isolates were deposited to NCBI. Phylogenetic analysis (Neighbor-Joining) generated by 38 representative PPV sequences indicated that Bolu isolates were clustered with PPV-M isolates and separated from other strains, as in BLAST analysis. To our knowledge, this is the first report of PPV in Bolu. This study reveals the necessity to carry out more extensive surveys to prevent the PPV dissemination in Bolu and to identify the complete genomes of the obtained isolates to determine their genetic variation.All the PPV-infected trees were destroyed as a consequence.

In the face of climate change, the point of view of existing agricultural production systems is changing, and biological issues are gaining importance in terms of sustainable agriculture. Environmentally friendly biological solutions come to the forefront in the management of diseases and pests that put pressure on sustainable agriculture. It has been proven by many studies that plant growth promoting rhizobacteria (PGPR) have positive effects on plant growth, as well as reduce or prevent viral diseases. However, determining the targeted virus species, the PGPR strains used, or the plant strains tested requires a serious literature review and reading. In this study, which can be considered as a preliminary study, a database of PGPR and plant virus relations literature was created in a dynamic infrastructure that can be constantly updated, and a web interface was developed as an R/shiny application. Thanks to this preliminary study, which has examples in different fields, it is thought that it is possible for researchers to quickly access the literature on the subject, plan cost-effective projects, and identify the missing or potential points of the subject. According to the information in the database, it was determined that CMV was the most targeted virus, different strains of Bacillus and Pseudomonas genera were used the most as PGPR agent, tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) were the most tested plant species, and single applications were tested more. It is thought that this and similar studies will enable researchers to gain a quick perspective on the subject, facilitate the management of the information they have acquired, and contribute to effective planning in their new studies.

Climate change, the effects of which are becoming more evident day by day, is seen as a serious threat to sustainable agriculture. As a result of the diseases and pests being affected by climate change, it is inevitable that changes will occur in the state of plant health, which has an important place in sustainable agriculture. Evaluation and analysis of the literature is of great importance, as research and assessments focusing on the impact of climate change on plant health use current knowledge. In this study, which was carried out to contribute to the aforementioned subject, bibliometric analysis was performed on the indexed researches on climate change and plant health in the Web of Science (WoS) database according to the determined search query. Information on different subjects such as authors, countries, published journals, citations, sources used and keywords related to the studies were analyzed with the Bibliometrix package developed in R software and the data were visualized. Quantitative results were obtained on subjects such as prominent authors, journals, countries, and common keywords as a result of bibliometric analyses. According to the results, the importance of multidisciplinary studies is becoming more and more important. In addition, it is gaining popularity to benefit from technological developments in the face of changing and emerging needs in the processing of all kinds of information about climate change. This study was carried out to show that there is an alternative way to gain a general perspective on climate change-related issues in similar studies to be carried out. It is thought that these and similar bibliometric analysis studies can contribute to the execution of more successful studies, thanks to the information on different topics they provide.