Background: Dengue fever (DF) is the most rapidly spreading mosquito-borne viral disease in the world. In this decade it has expanded to new countries and from urban to rural areas. Limited awareness and nonsystematized health education programmes have contributed adversely to the increase in dengue incidence due to limited attention which has positively contributed to the increase in vector receptivity. The current study was conducted to ascertain the knowledge, attitudes and Preventive measures regarding Dengue fever among the students of various universities in Bangladesh. Study design: A cross-sectional survey was carried out to assess the Knowledge, Awareness and Preventive measures (KAP) among the students of various universities in Bangladesh. Methodology: Between August 2020 & November 2020, an epidemiological survey was conducted among 199 students of various universities throughout Bangladesh. We collected information on the socio-demographic characteristics of the respondents and their knowledge, attitude and Preventive measures regarding DF using a structured questionnaire. Majority of the questions were dichotomous (Yes/No type) and some questions were asked using five point Likert scale.  We then statistically compared their knowledge, attitude and Preventive measures in regard to socio-demographic characteristics of the respondents to identify possible causes of observed differences using SPSS version 23. Results: Total number of students from Diploma, Undergraduate (Honor's), Postgraduate (Master's) were 199 who had given voluntary consent and participated in this study willingly.  The sample comprised more males (56%) than females (44%), and almost 50% of respondents were from allied health science (Pharmacy, Microbiology, Public health, Botany, Zoology, Biotechnology, Biochemistry etc) background. Respondents had relatively good knowledge about aspects of Dengue fever. Out of 199 individuals interviewed, 97% had heard of DF. They had good knowledge score regarding dengue symptoms but comparatively moderate knowledge score regarding dengue transmission and management. The knowledge, attitude and Preventive measures level of study respondents was found statistically significant in relation to the academic attainment of the respondents. Conclusion: We recommend implementing mass educational programmes throughout the country in order to raise the awareness and to translate knowledge into sound practice to control dengue disease epidemics in Bangladesh.