In this study are presented the results of studies for oregano in 16 locations (sites) of Albania for the content of the quantity of oils and their fractions. Albania is the one of the important diversity centers for Oregano because of their adaptation to diverse agro ecological conditions as a result of natural selection. The design of the experiment was based on split plot methods. From the total samples collected, were selected 16 mostly widespread samples of natural populations of origano (Origanum vulgare L. sp. vulgare and sp. hirtum). Essential oil isolation from oregano was performed by hydro distillation. Identification of the components was made by comparing mass spectra of components in essential oils with those from Nist, Wiley and Adams mass spectra libraries. The results show that the total oil content varies from 3.45% to 0.1%. The populations of Oregano in the part of southern Albania have a higher amount of etheric oil compared with those of the North part. The higher content of carvacrol in 55.63%, of the samples was determined in sites of the southern part of Albania, while in the other two investigated area central and north part it was 55.63% and 48.49% respectively. The same indicators were realised also for content of linalool and thymol, while North area has sites with high content of caryophyllen-oxide and β-pinen 3. But, in all sites, the oils of thymol and carvacrolit were dominant.