No abstract

Golongan darah menjadi salah satu hal penting yang harus dipahami oleh setiap orang dan dapat dengan mudah diiketahui menggunakan pemeriksaan khusus. Pemeriksaan golongan darah didasarkan pada pola reaksi antara antisera dan darah yang merujuk pada sistem penggolongan darah mayor. Metode pemeriksaan goldar yang paling umum dan sederhana adalah menggunakan slide-test. Pentingnya dalam mengetahui golongan darah tidak hanya pada transfusi darah, tetapi juga penting untuk identifikasi penyakit yang berhubungan dengan sel darah merah. Kegiatan Pengabdian Masyarakat ini bertujuan untuk memberikan pelayanan pemeriksaan golongan darah sekaligus memberikan pemahaman pentingnya melakukan pemeriksaan golongan darah pada mahasiswa. Metode Kegiatan: Kegiatan ini dilaksanakan pada  tanggal 20 Desember 2021 dengan sasaran mahasiswa yang belum mengetahui golongan darah. Penentuan jumlah sampel berdasarkan teknik purposive sampling. Pemeriksaan golongan darah menggunakan sistem A-B-O dan rhesus dengan metode slide test. Data hasil pemeriksaan dianalisis dengan menggunakan Microsoft excel. Hasil pemeriksaan golongan darah dari 16 mahasiswa yang memenuhi kriteria inklusi didapatkan  hasil terbanyak yaitu golongan darah B sebanyak 6 orang (37,5%) dan  golongan darah paling sedikit adalah O dan AB masing-masing sebanyak 3 orang (18,75).  Pada pemeriksaan rhesus menunjukkan bahwa semua mahasiswa memiliki rhesus positif (100%). Mahasiswa memahami pentingnya melakukan pemeriksaan golongan darah dan diketahui mayoritas mahasiswa memiliki golongan darah B dengan rhesus positif.

Human immunodeficiency virus (HIV) is a one of crucial concern to blood safety because of their prolonged presence in the blood. Unsafe blood transfusion from infected donor contribute to spread the virus as well. Indonesia health ministry reported that HIV infection increase significantly in 2019 to 2021, and HIV case diagnose in population still way from the prediction in 2020, only 78.7% cases reported from 90% prediction case. A retrospective cross-sectional study was conducted and collected 51,351 blood donors during COVID-19 pandemic, 2020-2021. Blood sample analyzed using serological methods to detect the present of HIV antibodies. A reactive result of HIV screening from 21,260 donor in 2020 showed 12 donors reactive (0.00056%), while in 2021 from 30,091 donor showed 42 donors reactive (0.142899%) and showed a significant different from 2020 and 2021 (p-value <0.05). The number of reactive donors increase numerously from 2020 to 2021, while total donor also increased. The level of social restriction has contribution of HIV reactive case among blood donor and showed from two years of pandemic with p-value <0.05. A significant increase of HIV infection among blood donor and the effect of restriction level to prevent COVID-19 transmission are clearly show in this study.

Blood services is one of the health support systems which play important role for fulfilling blood stock, blood production, and distribution. Fulfilment of blood stock become the main focus of blood support unit in Indonesia. During the Covid-19 pandemic, Indonesia Government applied community activities restrictions enforcement which social distancing became the major reason for decreasing donor quantity in Bojonegoro while blood supply was needed from other units. In case of increasing blood donor, socialization programme held at Sumbertlaseh Village. This programme accommodated by Student of diploma programme of blood bank technology, undergraduate programme of ners, dan Blood Donor Unit Bojonegoro. The programme share information about the important and advantage of blood donor using various media, such as brochure, leaflet, and presentation. The results of the activity show that 78.26% of the community has good knowledge about blood donor.

One of promising bio-prospect as producer of an antibacterial compound is endophytic fungi that live in endemic plants. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi, an South Kalimantan endemic plant that has ethnobotanical utilisation in the pharmaceutical field. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disc diffusion assay. The minimum inhibitory concentration (MIC) was measured by the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage due to the effect of the extract. The antibacterial compounds then were detected using GC-MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequence. Among 13 isolates, endophytic fungi identified as Botryosphaeria rhodina AK32 able to produce antibacterial compounds that exhibited the highest activity and have a broad spectrum, moreover capable against resistant bacteria (MRSA) with 1.56% of MIC value for all of the test bacteria. AK32 ethyl acetate extract was inhibiting the cell wall synthesis and penetrate the outer membrane of bacteria. Based on GC-MS, antibacterial compounds of AK32 ethyl acetate extract were di-n-octylphthalate, phenol, 2-methyl-, 4-pentadecyne, 15-chloro-, benzeneacetonitrile, and benztriazole.