Propionibacterium acnes merupakan bakteri gram-positif berbentuk batang dan merupakan flora normal kulit yang ikut berperan dalam pembentukan jerawat. Sabun wajah lebih sering digunakan sebagai alternatif antijerawat karena telah dikenal masyarakat luas dan lebih praktis penggunaannya dan ekonomis. Penelitian ini bertujuan untuk mengkaji dan mengetahui sabun pembersih wajah antijerawat yang mengandung ekstrak daun beluntas yang paling efektif dalam menghambat pertumbuhan bakteri P. acne. Pada penelitian ini dilakukan penentuan nilai Konsentrasi Hambat Minimum (KHM) ekstrak daun beluntas terhadap bakteri P. acne selanjutnya dan formulasi sediaan sabun wajah ekstrak daun beluntas. Sediaan sabun wajah ini dilakukan uji mutu dan uji Lebar Daya Hambat (LDH). Hasil penelitian menunjukkan bahwa KHM ekstrak daun beluntas terdapat pada konsentrasi 5%. Sediaan sabun wajah ekstrak daun beluntas memiliki aktivitas antibakteri terhadap P. acne danyang paling efektif menghambat bakteri P. acne adalah formula 3 dengan konsentrasi 15% dengan rata-rata LDH sebesar 13,5 mm. Hasil dari uji hedonik menunjukan formula 1 lebih unggul dibandingkan dengan formula lainnya.

No abstract

Rice yellow stem borer (Scirpophaga incertulas Wlk) (rice YSB) is one of the most important insect pests in rice (Oryza sativa L.). Plant damage caused by the pest attack in all growth stages could significantly reduce yield. Cry toxins from Bacillus thuringiensis are known to be effective against rice YSB, therefore transgenic rice events cv Rojolele harbouring the cry1B::cry1Aa fusion genes driven by the maize ubiquitin promoter have been developed to improve rice resistance. To determine that the resistant traits has been stably inherited and expressed, in-planta and in-vitro bioassays of the T10 generation were performed on 6 independent transgenic rice events using the 1st instar larvae of rice YSB. In-planta bioassay was performed on 5-week-old rice plants grown in pots in a transgenic glasshouse, in 7 replicates. Each plant was invested with 20 1st instar larvae of rice YSB. The in-vitro bioassay was performed using the stems of the transgenic rice events against 10 1st instar larvae of rice YSB, in aerated Petri dishes in a culture room under room temperature, in three replicates. Results showed that the T10 generation of the transgenic rice events stably maintained the transgene integration and their resistance against the rice YSB.

This research observes and analyses the agronomic characteristics and yield component of six T8 generation of Rojolele transgenic rice lines (P8, Q20, U10, W3, X22, Y7) harboring Cry1B::Cry1Aa gene fusion conferring resistance to yellow stem borer (Schirphopaga interculas) compared to the nontransgenic Rojolele control plants. The experiment was done in a biosafety containment at the Research Centre for Biotechnology-LIPI in a (4.0 x 3.2) m2 pot with 50 cm spacing amongst lines and 25 cm between plants of the same line with 10 replicates each line. PCR analysis using Cry1B::Cry1Aa specific primer showed that all lines tested were transgenic as shown by the presence of 816 bp amplicon. The W3 transgenic line had the most similar agronomic characteristics to the Rojolele control plants in terms of plant height, leaf length, tiller numbers, stem diameter and flowering time. Yield components of the W3 line such as panicle length and total grains were also the closest to the Rojolele control plants compared to other transgenic lines. However, the number of empty grains was the highest. In general, the agronomic characteristics and yield component of all transgenic lines were less than of the Rojolele control plants.

No abstract