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An attempt to quantify the spectral response of the polychromatic light sources and drinking water samples has been presented in this research. This had been executed by an objective methods of image quality assessment, in which the criteria of error visibility (differences) between images had been applied theoretically and practically. The theoretical part had been presented here by image quality assessment which gave results match with that of the practical part resulted by lab investigation. Otherwise and in spite of its differences in physical and chemical properties, samples of drinking water with their existence percentage didn't make any variation in the spectrum of polychromatic light sources and hence the spectrum of the light sources remains unchanged.

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