There is great potential of using probiotics in aquaculture to increase growth rates and improve the nutritional status of the cultured animal.In this study has analyzed the growth rate of abalone fed pudding probiotic Vibrio strain Abn1.2 -Alg3.1-enriched soybean protein. Vibrio strains Alg3.1Rf R -Abn1.2Rf R is agarase enzyme producing bacteria. Growth performanced of normal abalone were evaluated and compared with abalone fed pudding probiotic diet and abalone fed pudding probioticenriched soybean protein. Dinitrosalysilic acid was used to measured in situ agarase activity and viable cell count was used to determine whether the probionts Alg3.1Rf R -Abn1.2Rf R can colonize the gastrointestinal tract of abalon. Abalone fed a diet pudding probiotic Vibrio strain Alg3.1Rf R -Abn1.2Rf R -enriched protein exhibited a increased growth rate compared to abalone fed standard diet under laboratory conditions. The number of culturable cell reisolated from abalone fed pudding probiotic for 14 days ranged from 10 6 -10 7 cfu/g material. Agarase activity in the abalone digestive tract was higher in abalone fed pudding probiotic compared to abalone fed unsupplemented pudding.

This study aims to determine the impact of price, facilities and quality of service, in part or in combination, on intention to stay again. This study is a post hoc study using a quantitative approach. The sampling technique used the probability sampling technique, i.e. simple random sampling, and sampled customers who stayed at The Singhasari Resort Batu more than once from a total of 100 respondents. Sampling was performed according to the Slovin formula. The results of this study show that (1) price has a positive and significant effect on the intention to stay again, (2) facilities have a positive and significant effect on the intention to stay again, and (3) quality of service. has a positive and significant effect. (4) Prices, facilities, and quality of service all have a positive and significant impact on revisit intentions at the same time.

Abstract. Sarkono, Faturrahman, Sofyan Y. 2010. Isolation and identification of lactic acid bacteria from abalone (Haliotis asinina) as a potential candidate of probiotic. Nusantara Bioscience 2: 38-42. The purpose of this study was to isolate, select and characterize lactic acid bacteria (LAB) from abalone as a potential candidate probiotic in abalone cultivation system. Selective isolation of LAB performed using de Man Rogosa Sharpe medium. LAB isolate that potential as probiotics were screened. Selection was based on its ability to suppress the growth of pathogenic bacteria, bacterial resistance to acidic conditions and bacterial resistance to bile salts (bile). Further characterization and identification conducted to determine the species. The results showed that two of the ten isolates potential to be developed as probiotic bacteria that have the ability to inhibit several pathogenic bacteria such as Escherichia coli, Bacillus cereus dan Staphylococcus aureus, able to grow at acidic condition and bile tolerance during the incubation for 24 hours. Based on the API test kit, both isolate identified as members of the species Lactobacillus paracasei ssp. paracasei.

This study aims to classify the image depth data Hand Posture. Hand Posture is a form of hand and movement used to communicate. Hand Posture is difficult to classify because various human hand objects are complex articulation objects. The model used in this study is Convolutional Neural Networks using the VGG-19 Net architecture. Based on the results shows an increase in the percentage of classification accuracy in each subject is 0.9976, 1.0, 0.9984, 1.0, and 0.9992 respectively.

Normal microbiota in the gastrointestinal tract has a mutual relationship with its host. This research aimed to know the morphological diversity of heterotrophic bacteria that live in the gastrointestinal tract of rice field eel and its potency as probiotic. Bacterial isolation has been done by cultivated of the sample in the nutrient agar medium and then observed colonies and cell morphology. Candidate probiotics of isolates were selected based on the capability of starch, protein and lipid hydrolysis, bacterial resistance to acidity and bacterial resistance to antibiotics. The result of this research showed there are 10 isolates and have various colonies’ morphological appearance. All of those isolates consist of 40% cocci, 30% small rod and each 10% long rod, streptococci, and spiral. As many as 8 isolates were gram-positive and 2 isolates was gram-negative. The motility test showed 70% of isolates were motile and 30 % non-motile. The results of casein hydrolysis, starch, and fate indicate that 70% of isolates were able to obtain Hydrolyze casein, that 60% of isolates were able to hydrolyze starch and that none of the isolates were able to obtain hydrolyzed fats. All of the probiotic candidates who were tested in isolation are resistant to all antibiotics tested. Thus, it said that not all discoveries meet the criteria as potential candidates.