This study aimed to observed the fibroblast cell description in incision wound of the domestic cat after treated by sugar and honey. Fifteen domestic cats (Felis domestica) were used in the research. The cats were divided into five treatment groups as follow, the negative control group (KK1) was normal wound healing (without any treatment), the positive control group (KK2) used Bioplacenton® ointment, group 1 (KP1) used sugar, group 2 (KP2) used honey, and group 3 (KP3) used a combination of sugar and honey. All preparations were given topically for 14 days. The sample collection of the skin was performed on the 4th, 9th, and 14th days. Histopathological preparation with hematoxylin-eosin staining was conducted on all skin samples. Treatment with a combination of sugar and honey showed a very high increase in fibroblast cells, and this also occurred in KP1, KK2 and KK1. Group treated with honey (KP2) showed low fibroblast cell level. This research revealed that the combination of sugar and honey showed a good influence on the process of fibroblast cell formation during the wound healing process.