To evaluate the organochlorine pesticide (OCP) contamination of Manzala Lake, its ecosystem was investigated during the winter season (December to March). The studied ecosystem components were water, sediment, aquatic weeds, and fishes in four locations. The samples were analyzed by gas chromatography with electron capture detector. Pollutant levels of total OCPs showed significantly high levels in the water areas of Round road (46.253 ng/ml), Port-Said Damietta road (19.301 ng/ml), followed by Bughas El-Rasoah (5.539 ng/ml), then Ashtoum El Gamel (natural reserve area now) (0.289 ng/ml). Organochlorines were detected in sediment only in Round road (3.359 μg/kg) and Port-Said Damietta road (0.171 μg/kg) by significant order while they were undetectable in Ashtoum El Gamel and Bughas El-Rasoah. Total OCPs in aquatic weeds ranged between 0.194 μg/kg in Port-Said Damietta and 0.026 μg/kg in Ashtoum El Gamel. While OCPs were 0.160 and 0.153 μg/kg in Round road and Bughas El-Rasoah, respectively. Concerning fish muscles OCPs were significantly higher in the Round road area (0.397 μg/kg) followed by the Port-Said Damietta road (0.258 μg/kg), and finally, Ashtoum El Gamel samples (0.126 μg/kg). The results revealed the direct relation for the accumulation of OCPs between studied ecosystem parameters at the Manzala Lake during the winter season. Results also demonstrated that fish samples collected from the Manzala Lake in the studied areas were contaminated with levels of organochlorines, not higher than the maximum permissible level recorded by FAO/WHO, and that the public is not at risk with fish consumption.

The objective of this work was to evaluate the pomegranate seeds powder (PSP) as an effective antioxidant in growing rabbit's diets to alleviate the adverse effects of organochlorine pesticides residues (OCPs) on growth performance, antioxidant status and physiological parameters of growing rabbits. A total of 120 unsexed weaned V-Line male rabbits (4 weeks of age) were assigned at random to five experimental groups. The control group was fed a diet free of PSP. The negative control was fed a diet free of PSP and OCPs, while the other empirical groups were fed the same diet supplemented with 500, 750 and 1000 g of PSP/100 kg diet. The results indicated that PSP contained content of vitamin C and vitamin E; 3.13 and 1.45 mg/100g, respectively. The content of total phenols was 0.27% and total flavonoids were 0.21 %. The accumulation of OCPs was concentrated in the rabbit`s liver, followed by the muscle. The control diet contaminated with Alpha Benzene hexachloride (αBHC), Delta Benzene hexachloride (∆BHC), dildren and methoxychlor values. Dietary supplementation of PSP at levels of 750 and 1000 g tended to improve average daily gain and feed conversion ratio compared to the control group. Rabbits fed diet containing 1000g PSP had the lowest mortality %. Edible giblets were significantly increased in the treated groups. The values of Hemoglobin, Packed cell volume, White Blood Cell, lymphocyte, total protein, globulin, higher density lipoprotein (HDL), Total antioxidant capacity (TAC) and glutathione peroxidase (GPx) were improved, but plasma low density lipoprotein (LDL) and total lipids levels were decreased in the treated groups. A significant decrease of plasma and liver malondialdehyde (MDA) level and significant increase in liver (GPx) and catalase (CAT) were observed in rabbits fed diets supplemented with PSP groups. Conclusively, dietary pomegranate seed powder supplementation improved growth performance and enhanced the antioxidant status. So it eliminated the adverse effects of oxidative stress induced by organochlorine pesticides of growing rabbits diets.

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Comparison of liquid chromatography instruments with single quadrupole and tandem mass spectrometry for trace level analysis: Aflatoxin m1 (afm 1) in white cheese,

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