One way to reduce dependence on the use of inorganic fertilizers is to substitute organic fertilizers. Organic fertilizers come from the process of degradation of organic matter. To speed up the degradation process, a bio activator containing local microorganisms (LMO) was added as a starter. This study aims to compare bio activators from various sources of local microorganisms as a starter for organic fertilizer production. Media for microbial growth in LMO during fermentation (NA and MEA), media for testing the potential of bacterial isolates from each bio activator (modified GPA, SMA, CMC, SA, and YEMA). Experimental research methods, including; fermentation of organic matter making up the bio activator and testing the potential of LMO isolates for each bio activator. The number of bacteria respectively in BA-1, BA-2, BA-3 and Control; 45x105; 48.3x105; 5.8 x105 and 1.09 x107 CFU/ml, while total yeast respectively; 13.3x105; 16.4x105; 5.3 x105 and 4.30 x107 CFU/ml. The highest total microorganisms were found in BA-2. Proteolytic, amylolytic, cellulolytic, fermentative (LAB), and nitrate potency were positive for all treatments. BA-2 has the highest potency for proteolytic index 6.0, fermentative (LAB) 5.0, and nitrogen-fixing bacteria 4.0, the highest amylolytic and cellulolytic potentials are on BA-1 and BA-3 with indexes 11.0 and 5.0. The three types of LMO contain specific microorganisms and have catalytic potential so they can be used as bio activators for the manufacture of organic fertilizers. BA-2 is the best bio activator in terms of total microorganisms.

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