Sea urchin is highly valued seafood. Its bioactive compound also has a potential as natural antibacterial agent. The aim of this research was to screen the antibacterial activity using cup-plate method, toxicity potential using brine shrimp lethality test (BSLT) method, bioactive compound using phytochemical method, and proximate composition by AOAC. This research was devided into two phases, the preliminary research was to determine the best body part of sea urchin showing antibacterial activity. Sea urchins were collected from Pramuka Island and extracted by meseration method. For the preliminary research, gonad extract showed the high antibacterial activity against E. coli and S. aureus at 1.83 ± 0.74 mm and 1.5 mm, respectively. The main research includes the determination of proximate composition, toxicity, bioactive compound, and antibacterial activity from the best body part of sea urchin. The proximate composition from gonad of sea urchin showed that water content 64.97 ± 0.08%; ash 2.72 ± 0.13%; lipid 19.73 ± 0.04%; protein 12.26 ± 0.3%, and 0.33 ± 0.17%, respectively. The detected bioactive compounds from the three different solvents of gonads extracts were steroid, triterpenoid and saponin. The result of lethal toxicity (LC50) from the three gonads extract was 471.861 ppm (n-hexane), 563.226 ppm (ethyl acetate) and 577.531 ppm (methanolic), respectively. Gonads ethyl acetate extracts showed the highest antibacterial activity than n-hexane and methanolic extract, its inhibition zone was 2.71 mm against S. aureus and 4.13 against E. coli. Keyword : Antibacteria, bioactive compound, D. setosum, sea urchin

Abstrak. Teripang merupakan salah satu komoditas perairan yang memiliki nilai ekonomis penting, yang diketahui memiliki potensi untuk dimanfaatkan sebagai nutrasetika. Potensi teripang di Desa Kakara belum dieksplorasi senyawa bioaktifnya. Penelitian ini bertujuan untuk menganalisis senyawa biokimia dan uji toksisitas ekstrak, serta mengetahui kandungan antioksidannya. Hasil analisis menunjukkan bahwa ekstrak l tiga jenis teripang yang berbeda masing-masing Holothuria atra, Stichopus horrens, dan Holothuria hilla mengandung senyawa bioaktif dari golongan Flavonoid dan Saponin. Hasil analisis Brine Shrimp Lethal Toxicity (BSLT) menunjukkan nilai A 629,19 ppm, B 739,86 ppm , C 681,78 ppm metanol ketiga jenis teripang uji masuk dalam kategori toksik. Analisis antioksidan menunjukkan hasil A 562, 59 ppm, B 968,78 ppm, ekstrak teripang dikategorikan tidak memiliki aktivitas antioksidan. (LC50) were 629,19 ppm, 739,86 ppm, 681,78 ppm, respectively, which means the three sea cucumber extract was toxic. The three sea cucumber extracts have no antioxidant activities against DPPH, where their IC50 value was 562, 59 ppm, 968,78 ppm. Abstract. Sea cucumber is known as one of the highly valued seafood who has nutraceutical potential. Its bioactive compounds have yet to explore in Kakara Island. This research aimed was to analyze bioactive compounds and toxicity from three different methanol extracts of Sea cucumber using the phytochemical method and the Brine Shrimp Lethal Toxicity method, respectively, and to analyze their antioxidant activities by DPPH method. The detected bioactive compounds from H. atra, S. horrens, dan H. hilla extracts were Flavonoid and saponin. The lethal toxicity values

Smoked tuna is one of traditional fisheries product in Tobelo, North Halmahera. Tobelos people like this product because of its smoke taste. In Tobelo, there are many producer who sell this product, but information about its quality was unknown. In this research, the researcher wants to know about microbiology aspect of smoked tuna from three traditional market in Tobelo. The result of this research showed that Total Plate Count (TPC) value was from 7,5x1015,35x102 APM/g; smoked tuna from modern Market showed the highest Staphulococcus sp. colony at 1,3x103 CFU/g. colony of Salmonella sp. showed negative value for three different market. The highest fungy colony was 2,5x101 CFU/g from Gotong royong Market, for Escherichia coli analysis, smoked tuna from Gotong Royong Market showed high value at 23 CFU/g, it was exceed Indonesia National Standard.

Generally each type of fish has different pattern of deterioration. Quality deterioration and high postharvest damage was caused by fishing method, poor handling process, the length of supply chain, poor handling facilities. This research was aimed to know about handling facilities on Purse seine board and its handling method; and to analyze fish quality using pH and organoleptic. The first step in this research was to observe the handling on the board then measure the pH of fish using pH metes. And also there was 30 panelist who did sensory test using organoleptic instrument on the board. The result showed that the handling facilities on board were coolbox, ice and hold, it showed that their apply good handling facilities. The average value of pH between 6,10 – 6,27, where the highest score was Ikan Selar (Selar crumenopthalmus) and the lowest score was Ikan Layang (Decapterus macarellus). The result of organoleptic was between 8,62-8,91, it showed that the tested fishes were fresh.

Micro and small enterprises (MSEs) play a vital role in the sustainability of regional economic development. The fisheries and agriculture sectors are the two leading sectors in North Halmahera Regency, contributing around 23% of the region's Gross Regional Domestic Product (GRDP). The purpose of this study is to identify external and internal factors that influence the development of fisheries MSEs and to formulate a strategy for developing MSEs in the fisheries sector. The data was collected using interviews with 30 respondents and focus group discussions. The analysis used external factor analysis (EFAS), internal factor analysis (IFAS), and SWOT analysis to formulate a strategy for developing MSEs' players. The results of the interviews show fisheries MSEs face challenges such as lack of infrastructure, limited capital, and limited competence in the fishery process. According to the IFAS results, the fisheries MSEs in North Maluku have a more significant weakness score than the strengths indicated by the score-0.49. Based on EFAS results, the fisheries MSEs in North Maluku have more significant opportunities than threats. The SWOT analysis indicates the MSEs in the fisheries sector in North Halmahera are in quadrant II, which supports a potential development strategy, including market development, market penetration, product development, and horizontal integration.