A nitrogen-fixing bacterium, designated strain X19-5 T , was isolated from rhizosphere soil of Sonchus oleraceus. Phylogenetic analysis based on a fragment of the nifH gene and the fulllength 16S rRNA gene sequence revealed that strain X19-5 T was a member of the genus Paenibacillus. Strain X19-5 T showed the highest 16S rRNA gene sequence similarity (98.8 %) with Paenibacillus graminis RSA19 T and below 97 % similarity with other recognized members of the genus. The level of DNA-DNA relatedness between strain X19-5 T and P. graminis RSA19 T was 45.7 %. The DNA G+C content of strain X19-5 T was 46.8 mol%. The major fatty acids were anteiso-C 15 : 0 , C 16 : 0 and iso-C 16 : 0 . On the basis of its phenotypic characteristics and the level of DNA-DNA hybridization, strain X19-5 T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus sonchi sp. nov. is proposed. The type strain is X19-5 T (5CCBAU 83901 T 5LMG 24727 T ).
A simple and efficient synthesis of N-sulfonyl ketimines through a Pd-catalyzed cross-coupling reaction between organoboronic acids and tosylbenzimidoyl chlorides under mild conditions has been developed.
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