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The purpose of this study was to determine the ability of aloe vera leaf extract (Aloe vera L.) in inhibiting the growth of Candida albicans fungi through in vitro. Aloe vera (Aloe vera L.) is a plant that can overcome digestive problems caused by fungi. One of the pathogenic fungi causing gastrointestinal infections is C. albicans. This research was conducted in Microbiology Laboratory of FMIPAUnsyiah which lasted from April to May 2010. A Completely Randomized Design (RAL) consisted of 5 treatments and 5 replications is used in this study. The treatment was arranged on P0 = 20 μl distilled water, P1 = aloe vera leaf extract concentration 100%, P2 = aloe leaf extract concentration of 50% and P3 = aloe leaf extract concentration of 25%. Aloe vera leaf extract (Aloe vera L.) was given to each treatment at 20 μl. The parameter observed was the diameter of the inhibit zone formed. The data were analyzed using Variant Analysis (ANOVA), then continued with the Smallest Significant Difference (BNT) test. The results of this study indicated that the presence of aloe vera leaf extract (Aloe vera L.) could inhibit the growth of C. albicans. The higher the concentration of aloe vera leaf extract (Aloe vera L.), the higher the ability of it to inhibit C. albicans. The diameter of the inhibitory zone formed will enlarge along with the increasing of the concentration of aloe vera leaf extract (Aloe vera L.). The extract concentrations of 100%, 50% and 25% had their resistance capability of 0.50 mg, 0.24 mg and 0.20 mg of nistatin antibiotics.

Studies related to the comparison of ectoparasites that infect snakehead from different habitats and their relationship to biometric conditions have not been widely studied. Thus, present study aimed to investigate the prevalence, intensity, dominance, and predilection of ectoparasites on snakehead collected from ditches, paddy fields, and swamps and correlate them with biometric conditions. In total of 90 snakehead fish were collected from ditches, paddy fields, and swamps. The observation of ectoparasites was performed on the gills, fins, and skin. The parameters measured in this study were ectoparasite profiles and biometric condition of fish. Specifically, the parameters of the ectoparasite profile included prevalence, intensity, dominance, and predilection. Meanwhile, the parameters of the biometric conditions were the length-weight relationship, the distribution of length and weight classes, and condition factors. Five species of ectoparasites that have been identified as Tetrahymena sp., Epistylis sp., Trichodina sp., Dactylogyrus sp., and Gyrodactylus sp. Tetrahymena sp. infection in snakehead was reported for the first time. The ditch habitat had the highest prevalence and intensity, which were 76.7% and 15.4 ectoparasites/fish, respectively. Tetrahymena sp. and Epistylis sp. were detected in sneakhead from all habitats, Trichodina sp. was detected at ditch and paddy field habitats, whereas, Gyrodactylus sp. and Dactylogyrus sp. were only found in swamp habitats. The gill was the predilection organ that most vulnerable to ectoparasite infection. Infected Snakehead tend to have lower average weight and length than healthy snakehead. Snakehead with a weight range of 115.2-145.2 g and a length range of 258.5-268.5 mm tend to be more vulnerable to ectoparasite infection compared to other sizes.Keywords:Tetrahymena sp.PrevalenceIntensityPredilectionLength-weight relationship

Bakteri pengikat nitrogen adalah jenis bakteri yang paling banyak ditemukan di tanah dan sebagiannya bersimbiosis dengan akar tumbuhan. Bakteri ini mengikat nitrogen di udara dan mengubahnya menjadi nitrat yang kemudian digunakan oleh tumbuhan. Media Jensen adalah media selektif yang umumnya digunakan untuk menumbuhkan bakteri pengikat nitrogen. Penelitian ini bertujuan untuk mengisolasi bakteri pengikat nitrogen dengan menggunakan media Jensen. Bakteri diisolasi dari rizosfer di sekitar UIN Ar-Raniry, Banda Aceh dan dilakukan karakteristik morfologi dan Optical Density (OD). Empat isolat (IS-A, IS-B, IS-C, IS-D) ditemukan pada serial pengenceran 10-2 dan 10-4 dan isolat D (IS-D) memiliki nilai Optical Density (OD) tertinggi yaitu sebesar 0,373.

Isolasi bakteri pengikat nitrogen perlu dilakukan pada berbagai ekosistem. Tanah gambut merupakan ekosistem dengan keragaman mikroba yang cukup tinggi. Penelitian ini bertujuan untuk mendapatkan isolat bakteri pengikat nitrogen dan karakterisasinya dari tanah gambut Kecamatan Trumon Aceh Selatan. Sampel tanah diambil dari empat stasiun dengan tipe tanah gembur dan liat, kedalaman bervariasi mulai 0-15 cm, 15-30 cm, 30-50 cm. Isolasi dilakukan dengan metode cawan sebar menggunakan media Jensen. Terdapat 25 isolat bakteri pengikat nitrogen, koloni berwarna putih transparan dan merah muda. Bentuk sel coccus dan basil. Berdasarkan hasil pewarnaan gram 9 isolat gram negatif dan 16 isolat gram positif. Hasil uji biokimia terhadap 14 isolat terpilih diperoleh pada uji Triple Sugar Iron Agar sebanyak 11 isolat memfermentasi glukosa dan 3 isolat tidak mampu memfermentasi glukosa. Semua isolat BPN tidak mampu memproduksi H2S. Pengujian sitrat diperoleh 9 isolat bereaksi positif dan 5 isolat bereaksi negatif. Terdapat 12 isolat menunjukkan hasil positif pada uji katalase, dan 2 isolat bersifat negatif. Hasil uji motilitas 8 isolat bersifat motil dan 6 isolat non motil. Pada pengujian urease semua isolat bereaksi positif, hal ini menunjukkan semua isolat yang diperoleh berpotensi dikembangkan sebagai biofertilizier