A fluid simulation calculation method of the microfluidic network is proposed as a means to achieve the flow distribution of the microfluidic network. This paper quantitatively analyzes the influence of flow distribution in microfluidic devices impacted by pressure variation in the pressure source and channel length. The flow distribution in microfluidic devices with three types of channel lengths under three different pressure conditions is studied and shows that the results obtained by the simulation calculation method on the basis of the fluid network are close to those given by the calculation method of the conventional electrical method. The simulation calculation method on the basis of the fluid network studied in this paper has computational reliability and can respond to the influence of microfluidic network length changes to the fluid system, which plays an active role in Lab-on-a-chip design and microchannel flow prediction.
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