The metabolic syndrome is the cause of death around the world caused by diabetic mellitus. Binahong leaf is a kind of plant that is widely used to treat various diseases. This study aims to investigate the inhibitory activity of binahong leaves compound in inhibiting the aldose reductase which has role of converting glucose into sorbitol by docking simulation. The compound of binahong leaves consists of ursolic acid, vitexin, and oleonolic acid (ligand testing). These compound were taken from PubChem site, while aldose reductase enzyme (receptor) was obtained from the world protein bank with PDB 2HV5 code. This study incorporated in silica technique by using Auto dock vina software, Discovery Studio and Ligplot as visualization. The result of grid box optimization by redocking comparative ligand was 0.7Å RMSD. The docking result showed that the free Gibbs energy (∆G) of aldose reductase was (-11.7), Vitexin (-8.3), Ursolic acid (-7.7) and Oleonolic acid (-8.6). These value suggested that there was a stable inhibition reaction from the binahong leaves compound and the comparative ligand. Based on the Lipinski Rule, the composition of binahong leaves compound meets the Lipinski Rule criteria which means this medicine can be used orally except for vitexin and comparative ligands of zopolrestate which exceed the number of the atom.

Cataracts are one of the most causes of blindness in the world. Oxidative stress can form pathological conditions such as cataracts. This oxidative stress ability can be measured by the malondialdehyde (MDA) biomarker. Binahong leaves (Anredera cordifolia (Tenore) Steenis) are native plants from Indonesia that are used to treat various diseases including cataract treatment. Binahong leaf (Anredera cordifolia (Tenore) Steenis) has a high amount of flavonoids and is rich in antioxidants that can be used to treat cataracts. Objective. The purpose of this study was to assess the effect of binahong leaf extract on the levels of MDA in a goat lens with cataract-induced material. Method. As many as possible, 40 goat eye lenses were divided into several groups, namely, group I normal lenses as controls (glucose 5.5 mM), group II lenses were cataract induced with glucose concentration of 55 mM, group III lenses with glucose 55 mM + binahong leaf extract (100 μg/ml), group IV lens with glucose 55 mM + binahong leaf extract (200 μg/ml), and group V lens with glucose 55 mM + quercetin (positive control). Biochemical parameters measured in the lens homogenate are malondialdehyde lens morphology in all groups’ observations and comparisons made. Results. The results of the study found that the lens group with the addition of binahong extract showed more results transparency compared to lens groups induced by glucose concentrations of 55 mM). This shows that the diabetic cataract group experienced high oxidative stress due to the accumulation of sorbitol compounds derived from glucose which caused turbidity in the goat eye lens and increased levels of lens MDA. Binahong levels at concentrations of 100 or 200 can inhibit MDA production. Conclusion. Binahong (Anredera cordifolia (Tenore) Steenis) extract has the ability to inhibit the production of MDA levels. In glucose-induced goat lenses, binahong extract and quercetin show antioxidant and anticataract properties.

BACKGROUND: Cataract is a condition where the retina cannot capture images completely and causes blindness due to an imbalance of reactive oxygen species (ROS). These free radicals can be suppressed using antioxidants from several plants, one of which is binahong leaves. AIM: The purpose of this study is to analyze the potential of binahong leaves as an initial screening that can play a role in inhibiting the process of cataractogenesis. METHODS: By extracting binahong leaves with 60% ethanol, the extracts were tested for phytochemistry using the X technique and GC–MS using the Perkin-Elmer GC Clarus 500. The experimental process and all laboratory analyses were fully carried out from June to December 2019 at the Laboratory of Food Analysis and Yield Agriculture, Department of Agricultural Product Technology, Syiah Kuala University, Banda Aceh. RESULTS: Analysis of binahong leaf potential showed that binahong leaves contain flavonoids, steroids, phenols, alcoholics, terpenoids, and saponins. While the GC–MS analysis results showed that binahong leaves are rich in phytol and contain fatty acids. CONCLUSION: Binahong leaves have the potential to be anticataractogenesis because they contain compounds rich in antioxidants.

Abstrak: Katarak Merupakan Penyakit Yang Berdampak Pada Kebutaan. Obat Penurun Kadar Sorbitol Memiliki Efek Mencegah Transformasi Glukosa Menjadi Sorbitol Yang Didapatkan Hasil Positif Pada Katarak Diabetes. Penelitian Ini Bertujuan Dengan Menilai Gambaran Kekeruhan Lensa Dan Penangkapan Radikal Bebas Dari Ekstrak Daun Binahong (Anredera Cordifolia (Tenore) Steenis) Sebagai Anti Katarak Dengan Percobaan Pada Lensa Kambing. Penelitian Ini Merupakan Penelitian Eksperimental. Penelitian Ini Dilakukan Preparasi Material Dan Ekstrak Tanaman Preparasi Sampel Daun Anredera Cordifolia (Tenore) Steenis Dan Melakukan Inkubasi Lensa Yang Terbagi Kedalam 5 Kelompok (Lensa Normal Dengan Glukosa 5,5 Mm, Lensa Katarak Diabetes Dengan Glukosa 55 Mm, Lensa Katarak Diabetes Dengan Ekstrak Daun Binahong 100 Μg/Ml, Lensa Katarak Diabetes Dengan Ekstrak Daun Binahong 200 Μg/Ml, Lensa Katarak Diabetes Dengan Vitamin E. Analisis Sampel Dengan Menilai Kekeruhan Lensa, Pengujian Antioksidan Menilai DPPH Dan IC 50. Hasil Penelitian Setiap Lensa Kelompok Eksperimen Menunjukan Perubahan Transparansi Lensa Apabila Dibandingkan Dengan Lensa Normal Serta Nilai IC50 Pada Kelompok Lensa Katarak Diabetes Dengan Ekstrak Etanol Daun Binahong 200 Μg/M Cukup Tinggi Dibandingkan Dengan Kelompok Katarak Diabetes Dengan Vitamin E. Ekstrak Daun Binahong (Anredera Cordifolia (Tenore) Steenis) Menunjukkan Sifat Antioksidan Kuat.