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Objective: The aim of this study was to isolate the constituents and evaluate the antioxidant activity of Solanum rostratum Dunal (Solanaceae) from Algeria. Methods: In this study, phytochemical analyses of the chloroform, ethyl acetate, and n-butanol extracts obtained from the aerial parts of S. rostratum were performed by column chromatography, thin-layer chromatography, and high-performance liquid chromatography techniques. The antioxidant activity was performed by 2,2-diphenyl-1-picrylhydrazyl scavenging assay method.Results: The identification and structure elucidation of the isolated compounds were compared with their nuclear magnetic resonance spectra and the literature led to identify one monoterpene glycoside (linalyl-β-glucopyranoside) (1) and three flavonoid glycosides: Apigenin-7-O-glucoside (2), astragalin (3) and (isorhamnetin-3-O-glucoside (4).Conclusion: This is the first report on the isolation and structure elucidation of compound (1) (linalyl-β-glucopyranoside) and compound (2) (apigenin-7-O-glucoside) from this species. In addition, antioxidant effect of the chloroform extract from S. rostratum was evaluated. The chloroform extract exhibited the remarkable radical scavenging ability (IC50=0066±0.001 mg/mL).

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Ö ZB u çalışmanın amacı Satureja boisiseri Hausskn. ex Boiss.(Lamiaceae) uçucu yağının biyolojik aktivitelerinin araştırılmasıdır. Uçucu yağın büyük bileşiklerinin antimikrobiyal aktivitesi agar kuyu ve disk difüzyon yöntemleri ile belirlenmiştir. Uçucu yağın, insan kolorektal adenokarsinom (HT-29) hücre hattına karşı antiproliferatif etkisi Gerçek Zamanlı Hücre Analizörü xCELLigence yöntemi ile değerlendirilmiştir. Ayrıca, uçucu yağın toplam fenolik miktarı ve radikal giderim aktivitesi de tespit edilmiştir. Sonuçlar göstermektedir ki; timol, %40 timol : %60 karvakrol, %25 terpinen-4-ol: %75 karvakrol S. aureus ATCC 29213 suşlarına karşı önemli bir antimikrobiyal aktiviteye sahiptir. Bununla birlikte, uçucu yağ HT-29 hücre hattının büyümesi üzerine yüksek bir inhibisyon etki göstermiştir (IC 50 <10 µg/mL). Anahtar KelimelerSatureja boissieri, antimikrobiyal, antiproliferatif, HT-29. A B S T R A C TT he aim of this study was to investigate the biological activities of Satureja boissieri Hausskn. ex Boiss. (Lamiaceae) essential oil. Antimicrobial effects of the major components from the essential oil were determined by agar well and disc diffusion methods. Antiproliferative effect of the essential oil against human colorectal adenocarcinoma (HT-29) cell line was evaluated by Real Time Cell Analyzer xCELLigence method. In addition, total phenolic content and radical scavenging activity of the essential oil were determined. The results show that thymol, thymol 40%:carvacrol 60%, and terpinene-4-ol 25%:carvacrol 75% had significant antimicrobial activity against S. aureus ATCC 25923 and S. aureus ATCC 29213 strains. Also, the essential oil exhibited the high inhibitory effect on the growth of HT-29 cell line (IC 50 <10 µg/mL).