Mycobacterium tuberculosis is a cause of tuberculosis (TB) disease. In this research Mycobacterium tuberculosis can be detected using the Microscopic and Rapid Molecular Test methods. This research aims are to know the difference in the results of the examination Mycobacterium tuberculosis with microscopic method and Rapid Molecular Test. This research used an experimental research design and was tested using the Chi Square test. This results showed at significant difference (p=0.000) detection of pulmonary TB disease by microscopic and Rapid Molecular Test methods.