Rusa merupakan salah satu sumber daya genetik yang ada di Indonesia. Keberadaan populasi rusa timor semakin menurun sebagai akibat adanya perburuan liar untuk berbagai kepentingan. Usaha yang dilakukan agar populasi rusa di alam tetap lestari ialah dengan melakukan pengembangan rusa timor melalui konservasi ex situ. Penelitian ini bertujuan untuk mengetahui karakteristik morfologi rusa timor dan sistem pemeliharaan pada penangkaran di Kota Kupang. Sampel yang digunakan adalah 35 ekor rusa timor yang dipelihara pada dua penangkaran di Kota Kupang. Metode yang digunakan adalah metode observasi, wawancara, dan pengukuran menggunakan pita ukur meliputi: pengukuran panjang badan (cm), tinggi badan (cm), lingkar dada (cm), panjang ekor (cm), panjang telinga (cm), panjang kepala (cm), panjang ranggah (cm), dan bobot badan (kg) menggunakan rumus winter. Hasil penelitian menunjukkan adanya perbedaan antara rusa timor jantan dan betina. Secara morfologi, rusa timor jantan memiliki warna dasar kuning kecoklatan pada seluruh area tubuh dan tidak memiliki corak tertentu, sedangkan rusa timor betina memiliki warna coklat, dan berwarna coklat keabuan pada area ventral yaitu bagian kaki, perut, dagu dan bagian bawah leher. Secara morfometri, menunjukan adanya perbedaan nyata pada bagian-bagian luar tubuh rusa jantan dan betina yaitu : bobot badan, lingkar dada, panjang badan, dan tinggi badan. Rusa timor dewasa di Kota Kupang memiliki ukuran tubuh yang lebih besar dibandingkan rusa timor dewasa di Manokwari. Anak rusa timor di Kota Kupang memiliki ukuran tubuh yang lebih besar bila dibandingkan dengan anak rusa timor di Ciawi. Sistem pemeliharaan rusa timor pada dua kawasan penangkaran di Kota Kupang merupakan penangkaran semi terkurung (mini ranch) yang dipelihara di area pekarangan rumah.

African Swine Fever (ASF) is a viral disease that attacks pigs and to date has caused many pig deaths in Kupang Regency. ASF is caused by a double-stranded DNA virus from the Asfivirus genus and the Asfarviridae family. This research aims to determine the anatomical pathology of the swine landrace suspect ASF. Organ samples were collected from two male landrace pigs and two female landrace pigs, aged 7 months, from Oeltuah Village, Taebenu District and Tarus Village, Central Kupang District, Kupang Regency, NTT. Clinical examinations were carried out on sick animals that were found during the investigation, then necropsied on the dead animals were carried out and continued with anatomical pathology examinations at the Pathology Laboratory, Faculty of Veterinary Medicine, Nusa Cendana University. Anatomical pathology examinations are carried out by observing changes in the structure and appearance of the organs. The necropsy results showed sub-cutaneous ecchymosis hemorrhage in the abdomen, limbs and ears, gastric, intestinal and hepatic hemorrhage, hemorrhagic lymphadenitis in mesenteric lymph nodes, hyperemic splenomegaly, pteckie hemorrhage in the renal capsule,, multifocal hemorrhage in the renal medulla and pulmonary lobe. Based on the observation of clinical symptoms and changes in anatomical pathology, it can be concluded that the death of pigs was suspected to be caused by the suspect ASF.

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquat dichloride is harmful if enters to the body, causing Parkinson's disease, since it is disrupting dopamine production in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The study was done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway caused by paraquat dichloride treatment in Wistar rats as a model of Parkinson's disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group) were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquat dichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected once per week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg) and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline as prerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brains were removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry staining using tyrosine hydroxylase antibody. The results of staining then were observed under light microscope and analyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigra pars compacta in all treatment groups, however, the cell density were found decreased only in group IV. Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine 'Nigro striatal pathway' was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergic neurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.

Acalypha indica Linn is a tropical weed, grows annually in East Nusa Tenggara. The weed is member of Euphorbiaceae family, a largest plant family known as medicinal plant. The weed leaves were used by local people in NTT to treat wounds, diseases or myasis on their livestock. This study aim to investigate the secondary metabolites in A. indica L leaves as a scientific proven for its local use.  The extract preparation comprises of several steps, i.e collection of fresh leaves, dry and wet sortation. The clean leaves were air dried in a room temperature for about 2 weeks before grounded into powder and subjected to extraction. The extraction method was maceration with ethanol 96% as solvent. The dense extract was evaporated using rotary evaporator and subjected to phytochemical screening. The result shows that ethanol extract of A.indica leaves were tested positive for flavonoid and tannin. Alkaloid, saponins, triterpenes and steroid were tested negative on the extract.

Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth traits. Materials and Methods: Blood samples from 161 SO cattle were collected and analyzed. Deoxyribonucleic acid (DNA) was isolated. The DNA was electrophoresed and extracted, and finally, the annealing temperature was optimized, followed by amplification and sequencing. Next, we used a Basic local alignment search tool to assess the sequencing data. Results: The analysis revealed 22 single nucleotide polymorphisms (SNPs) in the MSTN gene in this region that showed genetic variation. Two SNPs, c.424 G > A, and c.467 G > C, were found to be significantly associated with SO cattle phenotypes of wither height, heart girth, and hip height (p < 0.05) but not with body weight or body length (p > 0.05). Conclusion: As a result of our findings, the MSTN gene polymorphism and its correlation with growth traits in SO cattle may be employed as a candidate marker in SO cattle and other beef cattle breeds.