Tooth extraction is a common thing, more often after extraction patients have any complication, to prevent it, physician provide some drugs from chemicals that often give adverse effects, so that required for a safe substitute medicine, which derived from herbs. One of the herbs are often used by people in the Mentawai Islands is a sarang semut's root species of Hypnophytum formicarum jack. This study aimed to determine the effect of sarang semut's extract orally for granulation tissue formation in wound healing after tooth extraction. This research is experimental with posttest only control group design. Subjects were 48-animal guinea pigs, were divided into 4 groups: group I (control 0.5% CMC, treatment group II, III, IV with 4,65mg, and 9.3 mg 6,2 mg dose tuber ethanol extract dissolved anthill in CMC 0.5%), were given 3 ml orally, 3 times a day until the day of decapitation. Early research conducted identification and phytochemical screening, this plant belongs to the species Hypnophytum formicarum jack, contain flavonoids, phenols, triterpenoids and tannins. The data was analyzed with non-parametric Kruskal-Wallis, to see the significance between doses used Mann-Whitney. The results showed that the extract of sarang semut effect (p < 0.05) on the formation of granulation system in wound healing after tooth extraction. The most effective concentration was 4.65 mg