Herbicides are increasingly applied in vineyards worldwide. However, not much is known on potential side effects on soil organisms or on the nutrition of grapevines (Vitis vinifera). In an experimental vineyard in Austria, we examined the impacts of three within-row herbicide treatments (active ingredients: flazasulfuron, glufosinate, glyphosate) and mechanical weeding on grapevine root mycorrhization; soil microorganisms; earthworms; and nutrient concentration in grapevine roots, leaves, xylem sap and grape juice. The three herbicides reduced grapevine root mycorrhization on average by 53% compared to mechanical weeding. Soil microorganisms (total colony-forming units, CFU) were significantly affected by herbicides with highest CFUs under glufosinate and lowest under glyphosate. Earthworms (surface casting activity, density, biomass, reproduction) or litter decomposition in soil were unaffected by herbicides. Herbicides altered nutrient composition in grapevine roots, leaves, grape juice and xylem sap that was collected 11 months after herbicide application. Xylem sap under herbicide treatments also contained on average 70% more bacteria than under mechanical weeding; however, due to high variability, this was not statistically significant. We conclude that interdisciplinary approaches should receive more attention when assessing ecological effects of herbicides in vineyard ecosystems.Electronic supplementary materialThe online version of this article (10.1007/s11356-018-2422-3) contains supplementary material, which is available to authorized users.

In a vineyard we examined the effects of broad-spectrum herbicides with three different active ingredients (glyphosate, glufosinate, flazasulfuron) on soil microorganisms. Mechanical weeding served as control treatment. Treatments were applied within grapevine rows and soil samples taken from there in 10–20 cm depth 77 days after application. Fungi were analyzed using classical sequencing technology and bacteria using next-generation sequencing. The number of colony-forming units (CFU) comprising bacteria, yeasts and molds was higher under flazasulfuron compared to all other treatments which had similar CFU levels. Abundance of the fungus Mucor was higher under flazasulfuron than glufosinate and mechanical weeding; Mucor was absent under glyphosate. Several other fungi taxa were exclusively found under a specific treatment. Up to 160 different bacteria species were found – some of them for the first time in vineyard soils. Total bacterial counts under herbicides were on average 260% higher than under mechanical weeding; however due to high variability this was not statistically significant. We suggest that herbicide-induced alterations of soil microorganisms could have knock-on effects on other parts of the grapevine system.

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Inter-rows in vineyards are commonly tilled in order to control weeds and/or to conserve water. While impacts of tillage on earthworms are well studied in arable systems, very little is known from vineyards. In an experimental vineyard, the impact of four reduced tillage methods on earthworms was examined: rotary hoeing, rotary harrowing, grubbing and no tillage. According to an erosion prevention programme, tillage was applied every other inter-row only while alternating rows retained vegetated. Earthworms were extracted from the treated inter-rows 10, 36, 162 and 188 days after tillage. Across dates, tillage methods had no effect on overall earthworm densities or biomass. Considering each sampling date separately, earthworm densities were affected only at day 36 after tillage leading to lower densities under rotary hoeing (150.7 ± 42.5 worms/m²) and no tillage (117.3 ± 24.8 worms/m²) than under rotary harrowing (340.0 ± 87.4 worms/m²) and grubbing (242.7 ± 43.9 worms/m²). Time since tillage significantly increased earthworm densities or biomass, and affected soil moisture and temperature. Across sampling dates, earthworm densities correlated positively with soil moisture and negatively with soil temperature; individual earthworm mass increased with increasing time since tillage. It was concluded that reduced tillage in vineyards has little impact on earthworms when applied in spring under dry soil conditions.

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