Dental implants are crucial therapeutic devices for successful substitution of missing teeth. Failure cases are mainly pathogen-associated events, allowing clinical progression toward peri-mucositis or peri-implantitis. The aim of this study was to compare the performance of two mechanical decontamination systems, Nickel-Titanium brush (Brush) and Air-Polishing system with 40 µm bicarbonate powder (BIC-40), by means of a novel bioluminescence-based model that measures microbial load in real time. Briefly, 30 disks were contaminated using the bioluminescent Pseudomonas aeruginosa strain (BLI-P. aeruginosa), treated with Brush (30 s rounds, for 90 s) or BIC-40 (30 s, at 5 mm distance) procedure, and then assessed for microbial load, particularly, biofilm removal and re-growth. Our results showed that Brush and BIC-40 treatment reduced microbial load of about 1 and more than 3 logs, respectively. Furthermore, microbial re-growth onto Brush-treated disks rapidly occurred, while BIC-40-treated disks were slowly recolonized, reaching levels of microbial load consistently below those observed with the controls. In conclusion, we provide evidence on the good performance of BIC-40 as titanium device-decontamination system, the clinical implication for such findings will be discussed. Author Contributions: Conceptualization, E.B., E.C., and F.L.; Methodology, A.M., F.L., and B.C.; Software, A.M., B.C., F.L., and E.C.; Validation, E.C., A.M., and E.B.; Formal analysis, B.C.; Investigation, F.L.; Resources, U.C.; Data curation, U.C. and E.B.; Writing-original draft preparation, A.M. and E.C.; Writing-review and editing, E.B. and U.C; Visualization, B.C. and F.L; Supervision, A.M., B.C., and E.B.; Project administration, E.C., and F.L.; Funding acquisition, U.C.