FACS). FACS analysis revealed CD8؉ T cells in the trigeminal ganglia by day 7, with more being present in 17-than 17 N/H-infected trigeminal ganglia (6.22% versus 3.5%) and a decrease in number through day 30 (2.7% to 1.2%). To detect apoptotic CD8 ؉ T cells, sections were assayed by TUNEL and stained for CD8 ؉ T cells. By day 7, ϳ10% of CD8 ؉ T cells in both 17-and 17 N/H-infected trigeminal ganglia had undergone apoptosis. By day 30, 58% and 74% of all T cells had undergone apoptosis in 17-and 17 N/H-infected trigeminal ganglia, respectively. Furthermore, no HSV strain 17-infected trigeminal ganglion neurons were apoptotic, but 0.087% of 17⌬Sty and 0.98% of 17 N/H-infected neurons were apoptotic. We conclude that the antiapoptotic effect of LAT appears to require the LAT promoter, with most of the antiapoptotic effect mapping within the StyI (؉447) to the HpaI (؉1667) region and a minor contribution from the upstream StyI (؉76) to StyI (؉447) region.

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