The aim of this study was to evaluate the influence of different manipulation techniques on the microhardness of two nanoparticulate restorative composites. Sixty-six specimens were prepared, which were distributed into 8 groups according to the restorative material (Filtek Z350 XT ® and Evolu-X ®) and manipulation technique: digital manipulation; digital manipulation and rubbing of gloves with 70% alcohol; glass plate manipulation; glass plate manipulation previously rubbed with 70% alcohol. Resin increments were inserted into a bipartite Teflon matrix with orifice (5mmx3mm) and photopolymerized with 750mW/ cm² laser apparatus for 40 seconds and stored in deionized water at 37°C for 24 hours. Specimens were then included in polyvinyl chloride rings for subsequent surface planing and microhardness testing (Vickers penetrator, 50g/15s). Data were submitted to descriptive and inferential statistical analysis (significance level of 5%). In groups where Filtek Z350 XT ® resin was used, hardness variation from 30 HV to 89.00 HV was observed, while for Evolu-X ® resin, the lowest averages were obtained in groups whose manipulation was digital with (59.27HV) and without (58.85 HV) use of 70% alcohol. Nanoparticulate composites, manipulated by different techniques, did not present a similar mechanical behavior when evaluated by the microhardness test. The highest hardness values were obtained for Filtek Z350 XT ® resin, manipulated in glove previously rubbed with 70% alcohol.