Three aquareovirus strains isolated from grass carp (Ctenopharyngodon idellus), geoduck clams (Panope abrupta) and herring (Clupea harengus) in North America and Asia were examined by RNA-RNA blot hybridization to determine their genogroup. The isolates from clams and herring were identified as members of genogroup A, but the isolate from grass carp did not hybridize to any of the known genogroups, suggesting that this virus probably represents a new, seventh genogroup.
Two urease-positiveVibrio spp. were isolated from a brown shark (Carcharhinus plumbeus) that died in captivity at a national aquarium. Morphological, biochemical, and molecular genetic studies revealed one of the isolates to beV. damsela; the other isolate was unique and has been classified asV. carchariae sp. nov. BothV. damsela andV. carchariae were found to be virulent for spiny dogfish (Squalus acanthias), causing death in less than 18 hours after intraperitoneal injection of ca. 4×10(6) cells.V. damsela was strongly cytotoxic for Y1 adrenal cell monolayers;V. carchariae exhibited weak cytotoxicity for Y1 cells.V. damsela contained cryptic plasmids and both isolates were urease positive.V. carchariae was able to utilize urea as sole source of carbon and nitrogen.
Although gamma interferon (IFN-y) exerts profound effects on the state of activation of macrophages, its influence on receptor-mediated phagocytosis and killing of extracellular bacteria is poorly understood. Human monocytes cultured in the presence of human recombinant IFN-,y exhibited an enhanced capacity to produce superoxide anion. Although these cells bound greater numbers of particles via Fc receptors, their capacity to phagocytose by these receptors or to bind or ingest particles via receptors for C3bi, mannose, or unopsonized Pseudomonas aeruginosa was substantially depressed in a dose-dependent fashion (0.1 to 1,000 U of IFN-,y per ml). Macrophage phagocytosis ofP. aeruginosa and Staphylococcus aureus opsonized with whole serum or with serum deficient in immunoglobulin or complement was also depressed. Macrophages cultured in the presence of IFN-y had a diminished capacity to kill both unopsonized and opsonized P. aeruginosa. We conclude that IFN-y inhibits macrophage nonopsonic and opsonic receptor-mediated phagocytosis and killing but enhances
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