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The increasing importance of artificial insemination during the past few years has emphasized the necessity for improved methods of semen evaluation. A number of methods for semen evaluatIon are of importance and among' these an estimation of sperm motility seems to be the most widely used. Ho,vever, this gives :10 criterion for determining the number of live and dead spermatozoa because a few very active sperm may produce the same apparent motility as a larger -number of less active sperm. Up to the present time there has been no definite method for determining the relative number of live and dead spermatozoa in semen samples. If such a method could be developed it would be of considerable importance in evaluating fresh and stored semen to be used for artificial insemination, as a criterion for studying methods of processing semen, and as an aid in determinin~ the degree of fertility in males. It is the purpose of this paper to present such a method and to demonstrate its accuracy.

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