Purification of staphylococcal ,B-hemolysin and its action on staphylococcal and streptococcal cell walls. J. Bacteriol. 89:378-389. 1965.-After growth of bovine-derived strains of Staphylococcus aureus in a completely dialyzable medium, the ,B-hemolysin in the culture supernatant fluids was purified by gradient-elution chromatography on cellulose phosphate. The purified hemolysin contained two components, demonstrable by immunodiffusion or electrophoresis, but was free from a-hemolysin, coagulase, A-hemolysin, enterotoxins A and B, autoclaving, 0.25% KCI, 0.25% NaCl, 0.15% K2HPO4, 0.05% MgSO4, 0.25% mannitol, and 0.5% L-arginine were added to the dialysate. The 378

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