Ocular conditions, anti-Leishmania antibodies and total protein of the aqueous humor were studied in dogs naturally infected by Leishmania (Leishmania) chagasi. Fifty dogs were analyzed and assigned into two groups of 25 animals each. All animals were submitted to routine ophthalmic exam. Results showed that 76% of the affected animals presented ocular signs, being uveitis the predominant. The mean of total protein in the aqueous humor of animals with uveitis was higher (P<0.05) when compared to the mean of animals with other ocular signs or no clinical sign. The anti-Leishmania chagasi antibody values in plasma were superior to those found in the aqueous humor (P<0.05).Keywords: dog, Leishmania (Leishmania) chagasi, aqueous humor, antibodies, protein RESUMO Estudaram-se as condições oculares, os anticorpos anti-Leishmania e os valores de proteína total no humor aquoso de cães infectados naturalmente por

The objective of the present study was to investigate the histopathological alterations and detection of parasites that occur in the eyes of dogs naturally infected with

Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection... Ciência

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The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries. L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated.