Background: Every day, more than 1,800 babies worldwide contract HIV from their mothers. Many of these cases occur in Africa including Ethiopia. The transmission of HIV from infected mothers to babies could occur during pregnancy, delivery and breastfeeding. For women to take advantage of measures to reduce transmission, they need to know about Mother To Child Transmission (MTCT) of HIV and their HIV status. The aim of this study was to assess the knowledge on MTCT and utilization of services designed for Prevention of Mother-To-Child Transmission (PMTCT) of HIV/AIDS among pregnant women.