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1995) ISBN 0-521-41088-6; 457 pages; $175.00Rather unusual for one to be asked to look at the second half of a two volume set, but it gives me the opportunity to say that I liked this so much that I have to expect the other to be both as exciting and emitting (if I can be forgiven the pun and can use this term to mean full of ideas and information). volume will soon lead to the conviction that one should take up botany, that autofluorescence can be a very good thing, that it would not be bad either to be a mineralogist or petrologist, that there is great deal to be learned about fluorescent staining (double meaning intended) and that a lot of it can be learned here. Induced fluorescence (e.g., via fixation), indirect fluorochromy (meaning immuno-and lectin-based methods), and enzymatically induced fluorescence all warrant separate chapters.The most amazing part of this book is the nearly 200 page section packed with really practical information (and another 50 odd pages of references). Huge numbers of fluorochromes are considered, with the information ranging from where to get them, excitation and emission maxima, structural formulae, and selected references illustrating how to use them. Of course, one can get slabs of this type of information from manufacturers' catalogues and, in particular, a guide to very recent special purpose fluorochromes from Molecular Robes, Inc. (which one would need in addition to this source to be up to date), but where else could one find out so much about so many and in such an unbiased form? Polymer Microscopy, 2nd Edition

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SUMMARY This paper describes an instrument for microspectrofluorimetry, based on the Leitz Microspectrograph. Important features are monochromatic epi‐illumination and photon counting.

SUMMARY This paper describes an instrument for microspectrofluorimetry, based on the Leitz microspectrograph. Important features are monochromatic epi‐illumination, a reference channel and a measuring system using photon counting with automatic recording of data in digital form. To minimize fading, measurements of fluorescence intensity can be made within as little as 200 msec from the commencement of irradiation.