The objective of the study was to determine the wound healing activity of the Clitoria ternate L. flower ethanolic extract in the gel preparation in a diabetic animal model. We conducted an optimization of the gel preparations from various concentration of carbomer (0, 5%, 1%, 1.5%, 2%) and triethanolamine (1%, 1.5%, 2%, 2.5%) through stability study. The best formula gel was used as a basis for the Clitoria ternatea L extract in the wound healing activity study. We divided the animals into six groups, namely negative (placebo), positive (bioplacenton® gel), test I (Clitoria ternate L flower extract gel 5%), test II (Clitoria ternate L flower extract gel 10%), and test III (Clitoria ternate L flower extract gel 15%), and normal control group. All of these groups were induced by alloxan (170mg/kg BW i.p) to make diabetic (>200mg/dL), except for the normal group. Afterward, these diabetic animals were made a 2cm long wound incision using a sterile scalpel on the back, then followed by the administration of the preparations twice a day depending of the groups for 14 days. The study showed carbomer 1% and triethanolamine 1.5% formula was gave the best gel formulation with fulfilled value of viscosity, pH, homogeneity, consistency, and organoleptic parameters. Moreover, the wound healing study showed Clitoria ternate L. flower ethanolic extract gel 15% exhibited the best wound healing activity in the diabetic animal model significantly (p<0.05) than negative, normal, and test II, but not significantly different with positive and test I (p>0.05). The complete healing time and wound healing average percentage for the negative, positive, test I, test II, test III, and normal groups were >14 days (undefined), 13 days, 14 days, 14 days, and 10 days, and 47%, 52%, 59%, 49%, 63%, respectively. The Clitoria ternate L flower extract gel 15% is very potential in topical medication for diabetic wounds.