One of the newest and most effective strategies to empower staff is building the infrastructure and platform for strengthening organizational learning. This study has conducted about the relationship between organizational learning and staff empowerment. This correlational study was conducted among the staff of a Children's Hospital affiliated to Tehran University of Medical Sciences, Iran in 2012. Numbers of 145 staff in this hospital has selected and enrolled by simple random sampling. The data collection tools were tow questionnaires for organizational learning and staff empowerment which approved their validities and reliabilities. Finally, collected data were analyzed by Spearman correlation test. Among the dimensions of organizational learning, knowledge transfer and integration with 3.47 and system perspective with 3.16 obtained highest and lowest scores respectively. Generally, overall rating of organizational learning and the staff empowerment variables were determined 3.25±0.71 and 3.74±0.59 respectively. Between the four dimensions of organizational learning (managerial commitment, system perspective, openness and experimentation, knowledge transfer and integration) and staff empowerment, was observed a significant correlation. According to the findings, the current situation of organizational learning and staff empowerment in studied hospital is relatively acceptable. It recommended that hospital systems can improve staff empowerment by developing organizational learning environment, creating experience and knowledge exchange platforms, using material incentives and specially focusing on the intrinsic motivation.