A sensitive and rapid routine method was developed which allows a general, unambiguous and reliable differentiation between fresh and frozen-thawed fish muscle. The suggested procedure consists of (i) mild extraction of muscle in isotonic and buffered medium, (ii) rapid separation of the marker isozymes-those of the mitochondrial aspartate aminotransferase-by cellulose acetate electrophoresis, (iii) specific staining at neutral pH for enzyme activity by sandwich with an agarose-substrate-chromogen pad, and (iv) a clearing and drying process to make a permanent record.
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