In this report we show that CD26 (dipeptidyl peptidase IV/DPP IV) is a novel molecular marker for differentiated thyroid carcinoma. Northern-blot analysis of 22 various thyroid tissues revealed that CD26 is a more specific marker of differentiated thyroid carcinoma than 3 proto-oncogenes previously reported to increase mRNA expression in thyroid carcinomas: c-met, c-erbB-2 and EGF-R. A comparative study of 3 CD26 assays, Northern blotting, immunohistochemical staining and activity staining clearly showed that CD26 enzyme activity staining is the most specific assay for differentiated thyroid carcinoma, yet the easiest to perform. Activity staining of 216 thyroid tissues detected CD26 in all 52 papillary carcinomas and all 5 follicular carcinomas, while all 58 cases of Graves' disease were CD26-negative. Among benign neoplasms, 54 of 55 adenomatous goiters and 29 of 33 follicular adenomas were CD26 negative. Staining intensity of the enzyme activity was relative to the degree of CD26 mRNA expression. Southern-blot study showed no gene amplification or major translocation of the CD26 gene in 7 papillary carcinomas examined. Based on this study, ectopic expression of CD26 in differentiated thyroid carcinomas is thought to be mainly caused by increased CD26 mRNA expression. In conclusion, CD26 activity staining is a simple, specific assay which should be added to the usual pathological examinations in order to distinguish differentiated thyroid carcinomas from benign thyroid diseases.

No abstract

Abstract.This paper reports the results of research to examine the possibility of using

The herbicide, pentachiorophenol decomposes within a few weeks after its application in rice fields. A reductive dechloronation was revealed as one of its decomposition pathways, in which some microorganisms play a dominant role and other soil chemical factors in a reduced condition are relatively of little importance. The stable decomposition products found in the paddy soil were 2, 3,4,5-, 2,3,5,6-and 2,3,4,6-tetrachlorophenol, 2,4,5-and 2,3,5-trichlorophenol, 3,4-and 3,5-dichlorophenol, and 3-chlorophenol.

No abstract