By measuring the ultrasonic propagation velocity, it can be concluded that CDR application has an ability to promote bovine enamel remineralization.

The aim of this study was to use ultrasonography to evaluate the effect of the self-assembling peptide P11-4 on acid erosion prevention. Curodont Repair (CR), which includes peptide P11-4, was used. Rectangular prisms of bovine enamel (4×1×1 mm) were immersed in pure orange juice for a period of 5 minutes six times per day for 28 days. These samples were divided into four groups of six specimens each and treated differently for an additional period of 28 days: 1) baseline group specimens were stored in artificial saliva; 2) CR group specimens were exposed to curodont without acid challenge; 3) NCRA (no curodont+acid challenge) specimens were treated with orange juice without curodont exposure; and 4) CRA (CR+acid challenge) specimens were treated with curodont before treatment with orange juice. The propagation time of longitudinal ultrasonic velocity (UV) was measured. Ultrastructural observation of each tested enamel surface was carried out using field-emission scanning electron microscopy (SEM). The UV data were analyzed using two-way analysis of variance with time and treatment as confounding factors. Post hoc pairwise tests among groups were performed using the Tukey honestly significant difference test. The average UV in intact bovine enamel for the baseline group ranged from 4,483 to 4,549 m/s and did not vary significantly within the test period. The average ultrasonic velocity (UV) in all samples decreased after the initial erosion. The UV in NCRA decreased further over time. Increased UVs were found for CR and CRA. For CR and CRA, there was no significant difference in UV at the end of the experiment from the initial value before erosion. In the results of SEM observation, the CR and CRA groups had similar morphologic features in that etching patterns were not clearly due to precipitation between the enamel rods. From the results of this in vitro study, it might be concluded that applying enamel matrix derivatives and self-assembling peptides on erosive lesions can improve remineralization.

This study investigated the effect of a coating material containing S-PRG fillers on the demineralization of bovine enamel and dentin, by measuring changes in the transmitted ultrasonic velocity. Bovine enamel and dentin specimens with and without coating (n=6 for each condition) were cut into blocks, immersed in 0.1 M lactic-acid buffer solution (pH 4.75) for 10 min twice daily throughout the test period, and stored in a demineralising artificial saliva solution (pH 7.0) between treatments. The propagation time of longitudinal ultrasonic waves was measured by a pulser-receiver with a transducer. Data were evaluated using ANOVA followed by Tukey's HSD test (α=0.05). The sonic velocity decreased over time for specimens stored in demineralising solution; however, coated specimens had a significantly higher sonic velocity than those without coating. The coating material containing pre-reacted glass-ionomer fillers therefore appeared to reduce the demineralisation of enamel and dentin.

This study evaluated the effect of a calcium phosphate desensitizer on the demineralization of bovine dentin by measuring changes in transmitted ultrasonic velocity. Bovine dentin specimens with and without application of desensitizer were immersed in 0.1 M lactic-acid buffer solution (pH 4.75) 10 min twice daily throughout the test period, and stored in artificial saliva solution (pH 7.0) between treatments. The propagation time of longitudinal ultrasonic waves was measured by a pulser-receiver. Data were evaluated using one-way ANOVA followed by Tukey HSD test (α=0.05). The ultrasonic velocity decreased over time in specimens stored in demineralizing solution (3,785-3,462 m/s); however, desensitizer-applied specimens had a significantly higher sonic velocity (3,945-3,990 m/s) than those without application. The calcium phosphate desensitizer appeared to reduce the demineralization of dentin and occluded dentinal tubules.

Effects of a fluoride-releasing varnish containing surface reaction-type prereacted glass ionomer (S-PRG) filler on dentin demineralization were examined using optical coherence tomography. Bovine incisors that were sliced and treated with undersaturated 0.1 M lactic acid buffer solution (DE group). A thin film of varnish-containing S-PRG filler was applied before demineralization (PRG group). Control was maintained in artificial saliva. Using optical coherence tomography of selected locations on the dentin surface, peak intensity (dB) and width (μm) at 1/e 2 were obtained and integrated values calculated. Although alterations in integrated values were different in each group, there was a slight but not significant increase in those for the control group and a slight but significant increase for the De group. For the PRG group, integrated values were doubled seven days after experiment initiation, followed by a significant increase. Fluoride-releasing varnish containing S-PRG fillers prevented dentin demineralization as detected by optical coherence tomography.