Osteosarcoma (OS) is the most common malignant bone tumor. In cancer cells, autophagy is related to epithelial-to-mesenchymal transition (EMT). Although microRNA (miR)-506-3p has been demonstrated to act as a tumor suppressor in OS, its role in regulating the EMT process and autophagy remains unknown. The results showed that miR-506-3p directly inhibited the expression of sphingosine kinase 1 (SPHK1) in 143B and SaOS-2 cells. The invasive capability of OS cells was reduced following miR-506-3p mimics transfection, and restored when SPHK1 was overexpressed simultaneously. Further, miR-506-3p mimics initiated mesenchymal-to-epithelial transition (MET) – E-cadherin expression was upregulated, whilst vimentin and fibronectin were downregulated. The basal autophagy flux (LC3II/I) was suppressed by miR-506-3p mimics. The alterations induced by miR-506-3p mimics were partly reversed by SPHK1 overexpression or treatment of rapamycin. Meanwhile, treatment of SPHK1-transfected cells with 3-methyladenine inhibited EMT. The data suggest that miR-506-3p initiates MET and suppresses autophagy in OS cells by targeting SPHK1.

ObjectiveTo investigate the effect of additional semiautomatic intermittent pneumatic compression device (IPCD) in the prevention of deep vein thrombosis (DVT) of lower extremity in these patients undergoing major orthopedic surgery, when compared with the conventional graduated compression stockings alone.MethodsThe data of 112 patients undergoing major orthopedic surgery were retrospectively analyzed. 51 patients who ever received IPCD and graduated compression stockings during major orthopedic surgery were taken as the experimental group, and 61 patients who only received the conventional graduated compression stockings during surgery were taken as the observation group. The Doppler sonography was utilized to detect the presence of DVT and pulmonary embolism pre- and postoperatively. Besides, the mean and peak velocity of blood flow in femoral vein were recorded before and after surgery. And then, the comparisons between the two groups were made, respectively.ResultsWhen compared with the conventional graduated compression stockings alone, the intraoperative application of IPCD and stockings contributed the significant reduction of DVT (3.92%, 2/51 versus 9.84%, 6/61, X2 = 5.632, P = 0.034). In terms of the mean and peak velocity of blood flow in femoral vein, the postoperative difference was higher in the observation group than those in the control group (149.56 ± 26.35 versus 130.15 ± 22.56 mm/s, P < 0.05). With respect to perioperative blood loss, the difference between the two groups was statistically significant (800.5 ± 320.7 versus 950.1 ± 305.9 ml, P = 0.031).ConclusionsIntraoperative application of IPCD could promote blood circulation of lower limbs, and significantly decrease the incidence of potentially fatal DVT in patients undergoing major orthopedic surgery, when compared with the conventional graduated compression stockings.

Objective: To explore the application and analysis of image segmentation in the rehabilitation of patients with bone trauma during orthopedic nursing. Methods: Sixty patients with orthopedic trauma in our hospital were enrolled. The patients were divided into observation group and control group by four weeks. The control group was the traditional observation image, and the observation group was analyzed by image segmentation technology. Therefore, the two groups of patients are provided with nursing treatment, and finally, the progress of bone trauma recovery is periodically observed. Results: The degree of recovery in the observation group was 43% in the four-week period and 30% in the control group. It can be seen that the adaptive threshold algorithm makes orthopedic care more effective in image segmentation. Discussion: In medical imaging technology, different imaging devices can provide a variety of voxel information. The bone morphology information provided by CT images can make orthopedic care more targeted. Conclusion: Image segmentation technology provides a reliable predictive treatment solution in the nursing work, which solves the actual problem of the patient in the injury and the disease, so that the patient can recover faster. However, the expected research results of medical imaging technology research have guiding significance for the doctor's clinical diagnosis and preoperative and postoperative planning.

Objective: To investigate the application of predictive nursing guidelines in orthopedic care based on medical imaging and health statistics. Methods: 140 patients were divided into two groups: observation and control. The patients in the control group received routine nursing. The patients in the observation group received the guidance of predictive nursing on the basis of routine nursing. The nursing effects of the two groups were observed. Results: After the implementation of nursing interventions, the complication rate was 4.3% in the observation group and 20.0% in the control group. The difference between the groups was statistically significant (P < 0.05). The satisfaction rate was 97.1%, and the nursing satisfaction of the control group was 90.0%. The difference between the groups was statistically significant (P < 0.05). Discussion: Through observation of patient satisfaction and treatment, especially in orthopedics, patients are differentiated according to different diseases, and predictive care can effectively prevent complications. Conclusion: Predictive care provides preventive measures by comprehensively understanding and comprehensively assessing the patient's condition and developing care measures in a timely and effective manner.

Background: To analyze computed tomography (CT) and magnetic resonance Imaging (MRI) in examination diagnosis of sacroiliac joint disease of ankylosing spondylitis. Methods: Eighty patients with ankylosing spondylitis were randomly selected our experimental subjects, and CT and MRI examinations were performed on them to observe and analyze the diagnosis results of the two examination methods. Results: The detection rates of I and II for ankylosing spondylitis sacroiliac joint disease were prominently higher than those of CT (P < 0.05). Note that detection rates pertaining to MRI on ankylosing spondylitis, sacroiliac joint disease, erosion of the articular surface, and bone cysts under the articular surface were significantly higher than those of CT (P < 0.05). There exists no significant differences in the detection rates of widening or narrowing and joint ankylosis (P > 0.05). Conclusions: The detection rate of MRI in clinical diagnosis of sacroiliac joint disease of ankylosing spondylitis is higher than that of CT. It possesses excellent clinical promotion and application.