The bivalent(or)mixed whole cell vaccines and outer membrane protein vaccines of Edwardsiella tarda and Pseudomonas fluorescens is the common Gram negative bacterial pathogens associated with diseases of Indian major carps were evaluated for their efficacy in Cirrhinus mrigala (Ham.). The Cirrhinus mrigala fingerlings were vaccinated with bivalent vaccines from E. tarda and P. fluorescens with or without immunoadjuvant (Asparagus racemosus tuber powder extract)by immersion method. On various days of Post Vaccination, [30 & 60 dpv] the treated fish were challenged with virulent strains (Edwardsiella tarda and Pseudomonas fluorescens) and fingerlings were monitored for RPS, cumulative mortality and other pathological symptoms for 10 days. The fishes were attained 100% mortality within 7,days in non-vaccinated groups, whereas the vaccines helped to increase the survival rate after 30&60 days of post vaccination [dpv]. Upon challenge with mixed pathogen, a high relative percent survival was recorded in the OMP vaccinated groups with immunoadjuvant (92%), when compare to other vaccinated and control groups.