Two-dimensional 'H NMR spectroscopy of bovine eye lens &crystallin and its isolated a,, and CT" subunits rcvcals that these aBf(rcgates have short and very flexible Gtcrminal extensions of eight (a,,) and ten (a,,) amino acids which adopt littlc preferred conformation in solution, Total o-crystallin forms a tighter aggregate than the isolated aA and a,, subunit aggregates. Our resulls arc consistent with a micellc model for u-crystallin quaternary structure. The presence of terminal extensions is a general feature of those crystallins, a and /3, which form aggregates.

The effects of macromolecular nonideality and crowding on chemical equilibria, association reactions, and enzyme kinetics.

IN /3-lactoglobulin thanks the Engineering Staff of Western Electric Co., Winston-Salem, N. C., for design of the electrical circuitry of the fluorometer. The capable technical assistance of Mrs.Zuzana LaGrange was deeply appreciated.

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