The effects of ozone and storage temperature on the survival of Giardia lamblia cysts, the cause of human giardiasis, and Giardia muris, a parasite of mice, were compared. The viabilities of the cysts were similar over a period of 25 days of storage in the range of -6" to 37°C; the optimum temperature for their long-term survival was determined to be about 5°C. Ozone was extremely effective for inactivating cysts of both Giardia species, with G. muris being consistently more resistant than G. lamblia at pH 7 and 5°C and 25°C.
Giardia lamblia cysts were inactivated in water with ozone at pH 7.0 and 5 and 25 degrees C. The concentration-time products for 99% inactivation were 0.53 and 0.17 mg-min/liter at 5 and 25 degrees C, respectively. These products were significantly lower than those reported for chlorine.
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