Two experiments were conducted to evaluate the effects of increasing dietary SID Lys in lactation on sow and litter performance. In Exp. 1, a total of 111 primiparous sows (Line 241; DNA Genetics, Columbus, NE) were allotted to 1 of 4 dietary treatments on d 110 of gestation. Dietary treatments included increasing dietary standardized ileal digestible (SID) Lys (0.80, 0.95, 1.10, and 1.25%). During lactation, there were no differences in ADFI or sow BW at weaning (d 21), resulting in no differences in BW loss. However, backfat loss during lactation decreased (linear, P = 0.046) as SID Lys increased. There were no differences in litter weaning weight, litter gain from d 2 to weaning, percentage of females bred by d 7 after weaning, d 30 conception rate, farrowing rate or subsequent litter characteristics. In Exp. 2, a total of 710 mixed parity sows (Line 241; DNA Genetics) were allotted to 1 of 4 dietary treatments at d 112 of gestation. Dietary treatments included increasing SID Lys (0.75, 0.90, 1.05, and 1.20%). Sow BW at weaning increased (quadratic, P = 0.046), and sow BW loss from post-farrow to weaning or d 112 to weaning decreased (quadratic, P ≤ 0.01) as SID Lys increased. Sow backfat loss increased (linear, P = 0.028) as SID Lys increased. Conversely, longissimus muscle depth loss decreased (linear, P = 0.002) as SID Lys increased. Percentage of females bred by d 7 after weaning increased (linear, P = 0.047) as SID Lys increased in parity 1 sows, with no difference in parity 2 or 3+ sows. Litter weight at d 17 and litter gain from d 2 to 17 increased (quadratic, P = 0.01) up to 1.05% SID Lys with no improvement thereafter. For subsequent litter characteristics, there were no differences in total born, percentage born alive, stillborn, or mummies. In conclusion, our results suggest that increasing dietary SID Lys can reduce sow protein loss in lactation. The optimal level of dietary SID Lys required by the sow may vary based on response criteria and parity.