Ultraviolet-B radiation induces the accumulation of UV-absorbing pigments of the flavonoid type in the epidermal layer of rye seedlings. The content of pigments identified as isovitexin derivatives was about 4 times higher in leaves preirradiated for up to 24 h with long wavelength UV-B radiation as compared to control plants without any UV-B pretreatment. Leaves preconditioned in this way could prevent or reduce damage to photosynthetic function caused by short wavelength UV-B radiation. Photosynthetic activity monitored by variable fluorescence was reduced in unprotected leaves compared to protected leaves when expressed as a fluorescence decrease ratio value, as quantum yield of photosystem I1 (Fvm/Fm) or as leaf vitality index (FmiFo).
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