The nucleotide sequence of a cloned DNA coding for the 35-kDa polypeptide of the eye lens of the frog (Rana temporaria) has been determined. The sequence without connectors and poly(A) tract is 889 nucleotides in length and shows no homology with sequences coding for other classes of crystallins: cy-, &, y-or S-crystallins. The sequence contains one reading frame 675 nucleotides in length, an apparently intact 3'-non-translated region with the polyadenylation signal sequence and a poly(A) tract; the 5'-nontranslated region is lost along with part of the coding region; this accounts for about l/4 of the total mRNA length. The secondary structure prediction according to the Ptitsin-Finkelstein method shows the presence of predominantly P-strands with only a few a-helical regions. We conclude that the 35-kDa polypeptide from the frog eye lens belongs to a new class of eye lens crystallins for which we propose the name c-crystallin. Recombinant

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