Objective: The present work is based on the above pyrazoline derivative form heterocyclic substituted chalcone. Methods: The elemental analysis, IR, 1H and 13C NMR and mass spectrum analyses were used to characterise the structures of the obtained compounds. Results: The scheme-1 and scheme-2 describe the synthesis of chalcone (thiophene-fluorenyl derivative) and synthesis of substituted pyrazolines respectively. Chalcone has been synthesized from 2-Acetylfluorene, which had been reacted with phenylhydrazine, 4-substituted phenylhydrazine (4-Chloro and 4-Nitro) and 2,4-Dinitrophenylhydrazine to form the substituted pyrazolines (CP01-CP04). Conclusion: The antimicrobial and antioxidant properties were evaluated on those newly synthesised compounds.