The phytopathogenic fungus Alternaria eichorniae attacks water hyacinth, an economically significant aquatic weed. The novel phytotoxin alteichin was isolated from liquid cultures of this fungus and its structure was deduced by X-ray crystallographic analysis. Altheichin is a doubly hydrated form of 4,9-dihydroxy perylene-3, 10-quinone. A single step dehydration of alteichin to anhydroalteichin is catalyzed both by acid and by a crude enzyme preparation from water hyacinth.